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pLVX-EGFP-HOTag3

Plasmid

#225677

Purpose

Used to generate stable cell lines that express EGFP-HOTag3. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

Depositor

Likun Wang

Article

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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Pet28a-His-tag-BS2

Plasmid

#220347

Purpose

E. coli expression vector to purify BS2

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

His tag

Expression

Bacterial

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Human USP30 (1-517, WT, MG-38-11)

Plasmid

#110748

Purpose

Transient transfection of human USP30

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-I-SceI

Plasmid

#60960

Purpose

Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

I-SceI restriction enzyme

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pPSmOrange-N1

Plasmid

#31898

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-(empty)-TAG

Plasmid

#138209

Purpose

To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Type

Empty backbone

Use

T7 transcription

Tags

TAG sequence

Expression

Mammalian

Promoter

CMV Promoter, T7 Promoter

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 JellyOp

Plasmid

#41432

Depositor

Robert Lucas

Article

Bailes et al PLoS One. 2012;7(1):e30774. Epub 2012 Ja

Insert

Box jellyfish opsin

Tags

1D4 epitope at 3' end

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-SADB19L

Plasmid

#32632

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

SADB19L

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-SADB19N

Plasmid

#32630

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

B19N

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pAD

Plasmid

#64747

Purpose

Yeast expression plasmid: ACC1 (ser659ala, ser1157ala)

Depositor

Jens Nielsen

Article

Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

Insert

ACC1 (ser659ala, ser1157ala)

Expression

Yeast

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-mCherry (527)

Plasmid

#70719

Purpose

Expression vector for mCherry purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSUPER TBK1

Plasmid

#26210

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER TBK1 (TBK1 Human)

Use

RNAi

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCWU6-xylR-E-luciferase

Plasmid

#190721

Purpose

fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

Depositor

Chenggang Wu

Article

Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

Insert

xylR-E-luciferase

Use

Luciferase

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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VT028327-FRT10-STOP-FRT10-GAL4

Plasmid

#232829

Purpose

Enhancer VT028327 driven GAL4, gated by Sparse.FRT10

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJW1511

Plasmid

#62365

Purpose

pEF1a-puro-T2A-ATHmmuRPL10A

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

Puro-T2A-AVITEVHAmmuRPL10A

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pH6HTC_RAN-Halo

Plasmid

#175339

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

RAN (RAN Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHPRT-DRGFP

Plasmid

#26476

Purpose

DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

Insert

pHPRT-DRGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

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HA-p73α-pcDNA3

Plasmid

#22102

Depositor

William Kaelin

Article

Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

Insert

tumor protein p73 (TP73 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pYPQ132B

Plasmid

#69282

Purpose

Golden Gate entry vector; 2nd gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ131A

Plasmid

#69273

Purpose

Golden Gate entry vector; 1st gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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