We narrowed to 18,241 results for: Jun
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Plasmid#103151PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7d-5p target (MIRLET7D Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTet01
Plasmid#78283PurposemRFP1 expressed behind an ATc-inducible constructDepositorInsertTet inducible mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR3c-R248C
Plasmid#45712DepositorAvailable SinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pMG81-MBP-t-M.MpeI-N374K-His
Plasmid#197985PurposeExpresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.DepositorInsertMBP-t-M.MpeI-N374K-His
TagsHis and MBPExpressionBacterialMutationM.MpeI-N374KAvailable SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIE-TGFB2-H6
Plasmid#128500PurposeExpresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.DepositorAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7b-5p
Plasmid#103148PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7b-5p target (MIRLET7B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-TAPBPR-TM
Plasmid#178645PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
TagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHelper-T7IS1
Plasmid#140631PurposeExpresses ShCAST under the T7 promoter and an empty sgRNADepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-miR34a-P2
Plasmid#64786PurposeLuciferase expression driven by miR-34a promoter P2DepositorInsertmiR-34a promoter P2 (MIR34A Human)
UseLuciferaseAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-19a-3p
Plasmid#103323PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19a-3p target (MIR19A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-NpHR3.3-EYFP
Plasmid#137151PurposeViral expression of NpHR3.3-EYFPDepositorHas ServiceAAV8InsertNpHR3.3-EYFP
UseAAVMutationW179FPromoternEFAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187948PurposeER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertER50-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianMutation6 mutations, T371A, L384M, M421G, G521R, Y537S, N…Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Alpha MHC-DN.JNK2
Plasmid#51931Purposemammalian expression of dominant negative JNK2DepositorInsertDN JNK2 (Mapk9 Mouse)
TagsHAExpressionMammalianMutationdominant negative: the dual phosphorylation moti…Promotercardiac-specific α-myosin heavy chain 5.5 kb pro…Available SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGem-nHyPer:cAPX
Plasmid#84736PurposeSimultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cellsDepositorInsertsTagsNLS-SV40ExpressionPlantPromoterpFMV/p35SAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-Hs4EHP_AE
Plasmid#148588PurposeMammalian Expression of Hs4EHPDepositorInsertHs4EHP (EIF4E2 Human)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track HA PGC-1 alpha
Plasmid#14427DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseAdenoviralTagsHAExpressionMammalianMutationLast four amino acids of PGC-1a removed to make t…Available SinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)
Plasmid#99229PurposeMammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.DepositorInsertpr-mEos2
TagsLifeactExpressionMammalianMutationA69T for primed conversion applicationsPromoterCMVAvailable SinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGem-sHyper:sAPX
Plasmid#84738PurposeSimultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cellsDepositorInsertsTagsnuclear localisation signals from the coding sequ…ExpressionPlantPromoterpFMV/p35SAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187950PurposeecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only