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We narrowed to 16,006 results for: grna

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Showing: 8661 - 8680 of 16006 results

  2. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid
    #157981
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Publication
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157981-plasmid-map-sequence-id-309444

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  4. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid
    #139086
    Purpose
    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139086-plasmid-map-sequence-id-273882

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  8. lentiCRISPRv2-sgRNAs-del-ZNF91-BFP

    Plasmid
    #202823
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Publication
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete exon 1 within ZNF91 gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    U6 - two
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202823-plasmid-map-sequence-id-399643

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  10. sgRNA(MS2)_zeo insert c-MYC

    Plasmid
    #164636
    Purpose
    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64
    Depositor
    Günter Schneider
    Insert
    target sequence for c-Myc activation (MYC Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164636-plasmid-map-sequence-id-326094

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  11. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

    Plasmid
    #181789
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181789-plasmid-map-sequence-id-354816

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  13. pspCas9-ABE-3'UTR-sgRNA-2xMS2

    Plasmid
    #132554
    Purpose
    Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2
    Depositor
    Baisong Lu
    Publication
    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABEmax
    sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
    Expression
    Mammalian
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    132554-plasmid-map-sequence-id-259533

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  14. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid
    #159283
    Purpose
    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Human INS sgRNA
    Human INS guide RNA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159283-plasmid-map-sequence-id-311907

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  15. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid
    #133340
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA_gcrA
    Use
    CRISPR
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133340-plasmid-map-sequence-id-269542

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  20. PX458_SSRP1_2

    Plasmid
    #72372
    Purpose
    Encodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SSRP1 (SSRP1 Human)
    Use
    CRISPR
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72372-plasmid-map-sequence-id-134668

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Showing: 8661 - 8680 of 16006 results