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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC RMDN3

Plasmid

#227878

Purpose

Used for transfection of cells for expression of C-MYC RMDN3

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

RMDN3 (RMDN3 Human)

Tags

MYC

Expression

Mammalian

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFKM1

Plasmid

#64950

Purpose

source of KmR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-KmR-FRT

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-B2705-SABR-Backbone

Plasmid

#119051

Purpose

3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pT/Caggs-NRASV12

Plasmid

#20205

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

NRas (NRAS Human)

Use

Transposon

Mutation

G12V

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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HRE-luciferase

Plasmid

#26731

Purpose

Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

Depositor

Navdeep Chandel

Article

Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

Insert

Hypoxia Responsive Elements (3)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-D614 (C-flag)

Plasmid

#156420

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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4xmts-mNeonGreen

Plasmid

#98876

Purpose

In vivo visualization of mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

4xmts

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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HMA-APOBEC1-Nb

Plasmid

#226012

Purpose

For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

Depositor

Eugene Yeo

Article

Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

Insert

APOBEC1 (Apobec1 Rat)

Tags

His-MBP and antiRabbit-nanobody

Expression

Bacterial

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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eMscL WT

Plasmid

#107454

Purpose

eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

Depositor

Francesco Difato

Article

Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

Insert

bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)

Use

Adeno-associated viral

Tags

Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

Expression

Mammalian

Promoter

human synapsin 1

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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