159,238 results

1. TFORF0980

   Plasmid

   #141777

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF7 (KLF7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. NOVA1 eGFP

   Plasmid

   #61275

   Purpose

   GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   NOVA1 (NOVA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AID∆C-Cas9n-UGI-NLS

   Plasmid

   #198890

   Purpose

   Express AID(1-188) BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Actication induced deaminase (AICDA Human)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 189-198

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PL-SIN-EOS-C(3+)-EGFP

   Plasmid

   #21318

   Purpose

   Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EOS-C(3+) promoter region

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF1922

   Plasmid

   #141888

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB20 (ZBTB20 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNP1047

   Plasmid

   #222726

   Purpose

   pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3 Flag Jnk2a1

   Plasmid

   #13754

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk2a1 (MAPK9 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pET-26b-NbVHH05-HA-His

   Plasmid

   #171567

   Purpose

   Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-HA-His (UBE2J1 Human)
   

   Use

   Tags

   HA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABEmax-SpRY

    Plasmid

    #195279

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

    Depositor

    Carsten Lederer, Petros Patsali

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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