We narrowed to 15,909 results for: genome

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pORANGE Doc2A-GFP KI

Plasmid

#131478

Purpose

Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Doc2a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

Plasmid

#156442

Purpose

For transient expression of mouse PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5B (Pds5b Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_non-targeting

Plasmid

#140109

Purpose

CRISPR-Cas9 library validation (negative control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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AA145

Plasmid

#215937

Purpose

Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

Plasmid

#157986

Purpose

Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

Depositor

Lijia Ma

Article

Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

Insert

S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

Plasmid

#160623

Purpose

TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:dCas9:VPR-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3

Plasmid

#209025

Purpose

Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-Pom121-mScarlet

Plasmid

#171491

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

Pom121 (Pom121 Mouse)

Use

Pgehme

Tags

mScarlet

Promoter

T7

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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TRP1MX6-ins4

Plasmid

#195041

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

TRP1

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBLO2 casX

Plasmid

#123122

Purpose

E. coli expression vector for CasX sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX guide RNA

Use

CRISPR

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pX330 LC-FKBP-Cas9

Plasmid

#162724

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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AA025

Plasmid

#216005

Purpose

Fragmid fragment: (Cas protein) NG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9-NG_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Cas12j2

Plasmid

#173926

Purpose

Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-Phi29(+exo) (LM2759)

Plasmid

#208964

Purpose

Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Phi29(+exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AID'-BE4max

Plasmid

#174696

Purpose

Base Editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

AID'-BE4max (AICDA Human)

Expression

Mammalian

Mutation

UGI-E11K

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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PBLO CasX-gRNA

Plasmid

#126419

Purpose

E. coli vector for CasX gRNA --- Used for In Vitro Transcription

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

casX gRNA

Use

CRISPR; In vitro transcription

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ239

Plasmid

#108859

Purpose

FnCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

Cpf1 is rice codon optimized

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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AA299

Plasmid

#216029

Purpose

Fragmid fragment: (Cas protein) NNGG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Slug]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA296

Plasmid

#216028

Purpose

Fragmid fragment: (Cas protein) NNNNCC PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Nme2Cas9_v1.1 [Nme2]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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