Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 17,261 results for: tes

Clear keywords
Showing: 8701 - 8720 of 17261 results

  1. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    219823-plasmid-map-sequence-id-441127

    Enlarge

  2. pD649-HAsp-mLepr-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157617
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Publication
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    Lepr (Lepr Mouse)
    Tags
    Fc(DAPA)-Avi-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    157617-plasmid-map-sequence-id-381383

    Enlarge

  3. mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax

    Plasmid
    #173868
    Purpose
    Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)
    Depositor
    Takao Nakata
    Publication
    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
    Tags
    C-terminal CAAX-box and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173868-plasmid-map-sequence-id-341717

    Enlarge

  5. SOX2 KO (T2A-H2B-EGFP)

    Plasmid
    #167969
    Purpose
    SOX2 CDS replacement with T2A-H2B-EGFP
    Depositor
    Emma Rawlins
    Publication
    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SRY-Box Transcription Factor 2 (SOX2 Human)
    Use
    CRISPR; Donor template
    Tags
    H2B-EGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167969-plasmid-map-sequence-id-331127

    Enlarge

  8. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

    Plasmid
    #108484
    Purpose
    inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP
    Depositor
    Susanne Lens
    Publication
    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CenpB-dCEN-INCENP (INCENP Human)
    Tags
    EGFP and VSV
    Expression
    Mammalian
    Mutation
    CEN box replaced by CenpB 1-166
    Promoter
    CMV
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108484-plasmid-map-sequence-id-208768

    Enlarge

  12. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

    Plasmid
    #80272
    Purpose
    mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted
    Depositor
    Andrew Holland
    Publication
    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Plk4 (PLK4 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of multi-phospho degron (amino acids 282…
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80272-plasmid-map-sequence-id-151043

    Enlarge

  14. UCP3 promoter EP1

    Plasmid
    #71743
    Purpose
    Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activity
    Depositor
    Heinrich Taegtmeyer
    Publication
    Harmancey et al J Biol Chem. 2015 Nov 10. pii: jbc.M115.
    Insert
    Uncoupling Protein 3 promoter (Ucp3 Mouse)
    Tags
    Luciferase
    Expression
    Bacterial
    Mutation
    Contains 7kb of the murine UCP3 gene encompassing…
    Promoter
    UCP3
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71743-plasmid-map-sequence-id-130165

    Enlarge

  15. 2-Luc-Del-50

    Plasmid
    #21730
    Depositor
    Wafik El-Deiry
    Publication
    Zeng et al Nat Genet. 1997 Jan . 15(1):78-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p21 promoter deletion mutant (CDKN1A Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Identical to 2-Luc except that the 50-bp region b…
    Available since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  19. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

    Plasmid
    #179843
    Purpose
    Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP
    Depositor
    Xiaoping Bao
    Publication
    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCND2 (CCND2 Human)
    Use
    Luciferase and Synthetic Biology
    Tags
    eGFP and luciferase
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179843-plasmid-map-sequence-id-354943

    Enlarge

  20. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

    Plasmid
    #196086
    Purpose
    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA targeting lncRNA NORAD
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Cloned using SapI sites - destroyed after inserti…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196086-plasmid-map-sequence-id-388997

    Enlarge

Showing: 8701 - 8720 of 17261 results