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We narrowed to 17,264 results for: tes

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Showing: 8701 - 8720 of 17264 results

  4. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    219823-plasmid-map-sequence-id-441127

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  5. pD649-HAsp-mLepr-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157617
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Publication
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    Lepr (Lepr Mouse)
    Tags
    Fc(DAPA)-Avi-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    157617-plasmid-map-sequence-id-381383

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  6. mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax

    Plasmid
    #173868
    Purpose
    Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)
    Depositor
    Takao Nakata
    Publication
    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
    Tags
    C-terminal CAAX-box and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173868-plasmid-map-sequence-id-341717

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  8. SOX2 KO (T2A-H2B-EGFP)

    Plasmid
    #167969
    Purpose
    SOX2 CDS replacement with T2A-H2B-EGFP
    Depositor
    Emma Rawlins
    Publication
    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SRY-Box Transcription Factor 2 (SOX2 Human)
    Use
    CRISPR; Donor template
    Tags
    H2B-EGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167969-plasmid-map-sequence-id-331127

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  11. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

    Plasmid
    #108484
    Purpose
    inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP
    Depositor
    Susanne Lens
    Publication
    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CenpB-dCEN-INCENP (INCENP Human)
    Tags
    EGFP and VSV
    Expression
    Mammalian
    Mutation
    CEN box replaced by CenpB 1-166
    Promoter
    CMV
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108484-plasmid-map-sequence-id-208768

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  15. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

    Plasmid
    #80272
    Purpose
    mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted
    Depositor
    Andrew Holland
    Publication
    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Plk4 (PLK4 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of multi-phospho degron (amino acids 282…
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80272-plasmid-map-sequence-id-151043

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  17. UCP3 promoter EP1

    Plasmid
    #71743
    Purpose
    Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activity
    Depositor
    Heinrich Taegtmeyer
    Publication
    Harmancey et al J Biol Chem. 2015 Nov 10. pii: jbc.M115.
    Insert
    Uncoupling Protein 3 promoter (Ucp3 Mouse)
    Tags
    Luciferase
    Expression
    Bacterial
    Mutation
    Contains 7kb of the murine UCP3 gene encompassing…
    Promoter
    UCP3
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71743-plasmid-map-sequence-id-130165

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  18. 2-Luc-Del-50

    Plasmid
    #21730
    Depositor
    Wafik El-Deiry
    Publication
    Zeng et al Nat Genet. 1997 Jan . 15(1):78-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p21 promoter deletion mutant (CDKN1A Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Identical to 2-Luc except that the 50-bp region b…
    Available since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 8701 - 8720 of 17264 results