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pX029

Plasmid

#167152

Purpose

MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnH3H-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 SARS-CoV-2 S-frag6_nostop

Plasmid

#153184

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag6

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

June 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 S 24nt-del

Plasmid

#153952

Purpose

Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

June 23, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 S 24nt-del_nostop

Plasmid

#153953

Purpose

Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

June 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag2_nostop

Plasmid

#153174

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag2

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSRW1JZ

Plasmid

#129528

Purpose

C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

Depositor

Noelle L'Etoile

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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p426_Cas9_gRNA-R-RDS1a

Plasmid

#87405

Purpose

p426_Cas9_gRNA-RDS1a without the ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting RDS1a sequence ATTCAATACGAAATGTGTGC in yeast chromosome 3.

Depositor

Aindrila Mukhopadhyay

Article

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting RDS1a

Use

CRISPR

Promoter

ADH1, pTyrosine

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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PlepA

Plasmid

#55175

Purpose

Strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

lepA promoter

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pluc2-iRFP720

Plasmid

#98887

Purpose

Mammalian expression

Depositor

Laura Mezzanotte

Article

Mezzanotte et al Cell Transplant. 2017 Apr 26. doi: 10.37

Insert

Firefly Luciferase

Tags

iRFP720

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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mStayGold-LRRK2

Plasmid

#244977

Purpose

Express LRRK2 in mammalian cells with mStayGold tag

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

LRRK2 (LRRK2 Human)

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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HaloTag-LRRK2

Plasmid

#244976

Purpose

Express LRRK2 in mammalian cells with HaloTag

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

LRRK2 (LRRK2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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3xFLAG-FKBP-RILPL1

Plasmid

#244987

Purpose

Express RILPL1 in mammalian cells with 3xFLAG tag and FKBP

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

RILPL1 (RILPL1 Human)

Tags

3xFLAG-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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3xFLAG-LRRK2

Plasmid

#244986

Purpose

Express LRRK2 in mammalian cells with 3xFLAG tag

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

LRRK2 (LRRK2 Human)

Use

Lentiviral

Tags

3xFLAG

Promoter

CMV

Available Since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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HaloTag-JIP4

Plasmid

#244979

Purpose

Express JIP4 in mammalian cells with HaloTag

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

RILPL1 (RILPL1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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pAJF1126

Plasmid

#252905

Purpose

3'mmpL3(MSMEG_0250)-ALFA for mmpL3-ALFA in M. smegmatis via single crossover. Encodes hygromycin-resistance cassette

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

3' mmpL3(MSMEG_0250)-ALFA

Tags

ALFA tag

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO-Rabep1 shRNA 369958-CMV-TurboGFP

Plasmid

#254777

Purpose

Rabep1 shRNA 369958 in pLKO backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

shRNA 369958 (RABEP1 Human)

Use

Lentiviral

Promoter

CMV

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-Lyzc-mCherry-Rab4 DN

Plasmid

#254774

Purpose

Neutrophil specific Dominant Negative Rab4 in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rab4 (RAB4A Human)

Use

Zebrafish expression

Mutation

DN(S27N)

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-FL Rabep1-T2A-RFP-Caax

Plasmid

#254770

Purpose

Neutrophil specific Full length Rabep1 in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rabep1 (RABEP1 Human)

Use

Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-Lyzc-mCherry-Rab11 DN

Plasmid

#254775

Purpose

Neutrophil specific Dominant Negative Rab11 in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rab11 (RAB11A Human)

Use

Zebrafish expression

Mutation

DN(S25N)

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-RRV-ABE

Plasmid

#250716

Purpose

dLbCas12a-RRV based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-RRV with the linker of (GGGGS)x8

Depositor

Yiping Qi

Article

Cheng et al J Integr Plant Biol. 2026 Apr 2. doi: 10

Insert

ecTadA8e-(GGGGS)x8-dLbCas12a-RRV

Use

CRISPR

Tags

5' and 3' NLS

Expression

Plant

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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