We narrowed to 92,138 results for: lias

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AAVS1-Puro cTnT FUCCI

Plasmid

#136935

Purpose

donor plasmid for cardiac-specific FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Troponin T promoter

Use

Synthetic Biology

Expression

Mammalian

Promoter

cTnT

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBK-VRER-BE3

Plasmid

#85173

Purpose

Expresses VRER-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

VRER-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170809

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170810

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hUVRAG

Plasmid

#24292

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV irradiation resistance-associated gene (UVRAG Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
AAV-hRedOpsin-TGFB1

Plasmid

#164427

Purpose

AAV plasmid expressing TGFB1 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

Insert

Tgfb1 (Tgfb1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Nov. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2-MG-IRES2-mNeonGreen

Plasmid

#175508

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-EGFP-Bulged-miR-19

Plasmid

#91976

Purpose

EGFP reporter cell line with eight bulged miR-19 sites

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Plasmid EUPp_09

Plasmid

#157745

Purpose

PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

Depositor

Kang Zhou

Article

Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

Inserts

acetaldehyde dehydrogenase
alcohol dehydrogenase (ADH2 Budding Yeast)

Expression

Bacterial

Promoter

PgyrA

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

Plasmid

#228726

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

Plasmid

#228725

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

Plasmid

#228729

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

Plasmid

#228727

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

Plasmid

#228728

Purpose

Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R

Plasmid

#228730

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3_SEPT6

Plasmid

#71583

Purpose

mammalian expression of human SEPT6 with a GFP fusion

Depositor

Elias Spiliotis

Article

Hu et al Curr Biol. 2012 Jun 19;22(12):1109-15. d

Insert

Septin 6 (SEPTIN6 Human)

Tags

eGFP

Expression

Mammalian

Mutation

V206D

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-SpyCas9

Plasmid

#113033

Purpose

SpyCas9 expression in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SpyCas9

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GFPsm-myc.WPRE.SV40

Plasmid

#98926

Purpose

AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

GFP-smFP-myc

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFnCpf1GG

Plasmid

#131010

Purpose

Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of FnCas12a
GFP expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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