We narrowed to 14,443 results for: NTS

1. pUC8 human IFN-gamma

   Plasmid

   #17600

   Depositor

   Howard Young

   Article

   Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

   Insert

   interferon-gamma cDNA (IFNG Human)
   

   Expression

   Bacterial

   Available Since

   May 11, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pEGB 35s:dCas:BRD:tNos (GB1172)

   Plasmid

   #75401

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas9:BRD
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX62

   Plasmid

   #46854

   Purpose

   TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

   Depositor

   Monica Hollstein

   Article

   Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

   Insert

   attB-Puro-2A and Hupki p53 G245S (TP53 Human, Mouse)
   

   Expression

   Mammalian

   Mutation

   G245S

   Available Since

   Aug. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

   Plasmid

   #139990

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VQR=D1135V/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1 RPEL1-EGFP-3XNLS

   Plasmid

   #58469

   Purpose

   Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   MRTF-A RPEL1 (Mrtfa Mouse, Synthetic)
   3X NLS with 3X stop codons
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX68

   Plasmid

   #46857

   Purpose

   OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

   Depositor

   Monica Hollstein

   Article

   Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

   Insert

   attB-Puro-2A and Hupki p53 A138V (TP53 Human, Mouse)
   

   Expression

   Mammalian

   Mutation

   p53 A138V

   Available Since

   Aug. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. (564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

   Plasmid

   #163025

   Purpose

   Expression of CRISPRi with gRNA against mouse Pcsk9

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
   

   Use

   AAV

   Tags

   KRAB, SV40 pA, and myc NLS

   Expression

   Mammalian

   Promoter

   Alb-AAT

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

   Plasmid

   #139991

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VRER=D1135V/G1218R/R1335E/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.SF-iGluSnFR.A184V

   Plasmid

   #106199

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   GltI: A184V

   Promoter

   CAG

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-hMLH1dn (og codon)

    Plasmid

    #174823

    Purpose

    Mammalian expression of human MLH1dn (original codon usage)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (original codon usage) (MLH1 Human)
    

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC-C Sars-CoV-2 NSP12_P314L

    Plasmid

    #194813

    Purpose

    Sars-CoV-2 NSP12_P314L in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    NSP12_P314L (ORF1ab Sars-CoV-2)
    

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    P314L

    Available Since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV.hSynapsin.SF-iGluSnFR.S72A

    Plasmid

    #106176

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    GltI: S72A

    Promoter

    hSynapsin

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 155_KRAS in pmiRGlo

    Plasmid

    #78132

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    KRAS (KRAS Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    3'UTR containing miRNA binding sites

    Promoter

    PGK

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)_Granulin 4-no linker

    Plasmid

    #176925

    Purpose

    express Granulin 4 without linker 5 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 4 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSynapsin.SF-iGluSnFR.A184V

    Plasmid

    #106175

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    GltI: A184V

    Promoter

    hSynapsin

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG.SF-iGluSnFR.S72A

    Plasmid

    #106200

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    GltI: S72A

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. K785R Smarca4-V5 IRES-puro

    Plasmid

    #107059

    Purpose

    Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

    Depositor

    Jerry Crabtree, Courtney Hodges

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-puroR and V5

    Expression

    Mammalian

    Mutation

    K785R

    Promoter

    CAG

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCALNL-Rheb(S16H)

    Plasmid

    #194204

    Purpose

    Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.

    Depositor

    Angelique Bordey

    Article

    Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    S16H

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-pNFkB::Cre-mRuby3

    Plasmid

    #164137

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    NFkB promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Expression

    Mammalian

    Promoter

    NFkB synthetic promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.GFAP.SF-iGluSnFR.A184S

    Plasmid

    #106192

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    GFAP

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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