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PB_iNEUROD1_P2A_GFP_Puro

Plasmid

#168803

Purpose

PiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

NEUROD1 (NEUROD1 Synthetic, Human)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYLL201

Plasmid

#53454

Purpose

Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

Depositor

Leroy Liu, Lisa Lyu

Article

Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

Insert

HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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plenti guide Thy1.1

Plasmid

#128063

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Oskar Laur

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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HDAC7 Flag

Plasmid

#13824

Purpose

Mammalian expression of human histone deacetylase 7 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC7 (HDAC7 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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HA Nprl3 pRK5

Plasmid

#46330

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl3 (NPRL3 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCDH_blast_MCS_Nard_GFP_LAMIN

Plasmid

#167340

Purpose

Expresses GFP fused to lamin A (LA) in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1D(sfGFP)

Plasmid

#126963

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1C(sfGFP)

Plasmid

#126962

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1B(sfGFP)

Plasmid

#126961

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-SacB

Plasmid

#113635

Purpose

Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

Depositor

Miguel Valvano

Article

Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

Insert

sacB

Expression

Bacterial

Mutation

Please see Depositor Comments

Promoter

sacB promoter

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET24a isPETase

Plasmid

#225759

Purpose

Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

isPETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMH-SFB

Plasmid

#99391

Purpose

Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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p426-SNR52p-gRNA.csr-1.Y-SUP4t

Plasmid

#68060

Purpose

gRNA for csr-1 locus in N. crassa

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

gRNA for csr-1 locus

Use

CRISPR; N. crassa, fungi

Expression

Yeast

Promoter

SNR52

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCiPS

Plasmid

#60011

Purpose

Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.

Depositor

David Russell

Article

Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

Insert

Pol

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSP64T (DM#111)

Plasmid

#15030

Depositor

Douglas Melton

Article

Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

Insert

beta-globin 5' and 3' UTR

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S ET2K

Plasmid

#20927

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; SV40LT and Klf4 (SOX2, POU5F1, KLF4 Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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LCV2 STING KO

Plasmid

#217445

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human STING

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting STING (STING1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB554

Plasmid

#74884

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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SMARCA2-GFP

Plasmid

#65390

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA2 (SMARCA2 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Kras G12C

Plasmid

#58901

Purpose

Expresses human Kras with a G12C mutation

Depositor

Channing Der

Insert

KRAS (KRAS Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

G12C mutation

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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