We narrowed to 14,443 results for: NTS

1. HiBiT-FZD6

   Plasmid

   #195844

   Purpose

   Expresses human FZD6 with an N-terminal HiBiT; contains M345L

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD6 (FZD6 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   M345L

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR EGFR-SLP76-TagBFP

   Plasmid

   #188630

   Purpose

   Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   SLP76-TagBFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRT-DHX36iso1E335A

   Plasmid

   #159587

   Purpose

   Express N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells

   Depositor

   Markus Hafner

   Article

   Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   E335A

   Promoter

   CMV

   Available Since

   May 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES-hrGFP II-mTET1_CD

   Plasmid

   #83571

   Purpose

   Expresses a mutant catalytic domain of TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   TET1 amino acids 1-1417 deleted, catalytic domain…

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 416Gal-FUS-YFP

   Plasmid

   #29593

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   YFP

   Expression

   Yeast

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

6. Lenti-pHSE::Cre-mRuby3

   Plasmid

   #164136

   Purpose

   Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   HSE promoter-Cre-T2A-mRuby3
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Expression

   Mammalian

   Promoter

   HSE synthetic promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

   Plasmid

   #188634

   Purpose

   HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Human, Synthetic)
   

   Use

   CRISPR

   Tags

   CD3ε-mNeonGreen

   Expression

   Mammalian

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist-SARS-CoV-2 Δ18 B.1.351v2

   Plasmid

   #169463

   Purpose

   Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 416Gal-FUS-P525L-YFP

   Plasmid

   #29628

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   P525L

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pTwist-SARS-CoV-2 Δ18 B.1.1.7

    Plasmid

    #169459

    Purpose

    Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLAP-BUBR1

    Plasmid

    #114052

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1 (BUB1B Human)
    

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance)

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC-BAR-YR

    Plasmid

    #61766

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Bar gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Promoter

    trpC promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid

    #139995

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    HF1=N497A/R661A/Q695A/Q926A

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1072 pBabe puroL myr Akt K179M T308A S473A

    Plasmid

    #9015

    Depositor

    William Sellers

    Article

    DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

    Insert

    Akt1 K179M T308A S473A (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    K179M T308A S473A

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_mGFP-FGFR3-K508M

    Plasmid

    #191753

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1523A>T (p.K508M) + silent mutation c.1515A&…

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-MYO10-HMM

    Plasmid

    #87256

    Purpose

    Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST17-cDAXX

    Plasmid

    #169729

    Purpose

    Bacterial expression of N-terminally 6His tagged cDAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    DAXX (DAXX Human)
    

    Tags

    6 His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

    Plasmid

    #106204

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S + mRuby3 fusion

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

    Plasmid

    #233039

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    

    Use

    AAV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-MTS-CA-c-Src-FLAG

    Plasmid

    #44654

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Tags

    FLAG tag and mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    changed Tyr 530 to Phe

    Promoter

    CMV promoter

    Available Since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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