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We narrowed to 17,337 results for: MUT

Showing: 1291 - 1320 of 17337 results

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pGL3-CMV-Cdc42 3'UTR mutant (position 577-599)

Plasmid

#51391

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

NM_001039802.1 (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

miR-29 binding site mutant (position 577-599)

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
-962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

Plasmid

#32735

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Tags

Expression

Mutation

-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

Promoter

-962 CCND1

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized to remove terminal 7 aa (QEEQEEY) (JB162))

Plasmid

#39308

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal 7 aa (QEEQEEY) removed

Promoter

CMV

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only
pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

Plasmid

#229743

Purpose

Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

LIR2 mutant WTFI -> ATFA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N1-KH12mut (T236D H237D K299D N300D)

Plasmid

#225454

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D K299D N300D)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

T236D H237D K299D N300D

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (P.2/B.1.1.248) - No RBD Mutations

Plasmid

#212385

Purpose

Encodes SARS-CoV-2 spike D614G,V1176F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D614G,V1176F (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

D614G,V1176F

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)

Plasmid

#206434

Purpose

Vasa 463-661 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Vasa 463-661 (vas Fly)

Use

Tags

EGFP

Expression

Insect

Mutation

F504E

Promoter

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A_T

Plasmid

#147636

Purpose

Insect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A (TNRC6C Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1mut-F961A_S

Plasmid

#147514

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-CIM1-F961A (gw Human, Nematode)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres_S

Plasmid

#147516

Purpose

Insect Expression of HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres (TNRC6A Human)

Use

Tags

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut_R

Plasmid

#147388

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut (gw Fly, Nematode)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A_R

Plasmid

#147391

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A (gw Fly)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-mut_R

Plasmid

#147385

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-CIM1-mut (gw Fly, Nematode)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-ChRWR/Venus(ATG mut)

Plasmid

#197891

Purpose

Can be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

ChRWR/Venus

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

EF1a

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP

Plasmid

#190039

Purpose

Expression of FIP200 mutant

Depositor

James Hurley

Article

Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

Insert

FIP200 (RB1CC1 Human)

Use

Tags

GST and MBP

Expression

Mammalian

Mutation

(641-779)deletion & 1363 RDKDLIES 1370 to GSS…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T

Plasmid

#183364

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4a (CASP4 Canis lupus familiaris)

Use

Retroviral

Tags

Myc

Expression

Mutation

H342D_K345M_K379T

Promoter

MESV

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Mdm2 mutant M3 (399-489)-IRES-GFP

Plasmid

#180490

Purpose

Role of Mdm2 variants in tumorigenesis

Depositor

Martine Roussel

Article

Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

Inserts

Mouse Mdm2 mutant
Mouse Mdm2 mutant

Use

Tags

Expression

Mammalian

Mutation

399-489

Promoter

MSCV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Mdm2 mutant M2 (299-489)-IRES-GFP

Plasmid

#180489

Purpose

Role of Mdm2 variants in tumorigenesis

Depositor

Martine Roussel

Article

Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

Insert

Mouse Mdm2 mutant

Use

Retroviral

Tags

Expression

Mammalian

Mutation

299-489

Promoter

MSCV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro

Plasmid

#176089

Purpose

EGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutations in Lys35 to Ala, Lys68 to Ala, and Lys7…

Promoter

CMV

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5-CASC3 siRes 110-480 188/218-mut

Plasmid

#158544

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 110-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 110-480, C-terminal deletion, F188D, …

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

Plasmid

#126755

Purpose

Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD

Plasmid

#73299

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Use

Tags

intein

Expression

Bacterial

Mutation

S1079N-E1083Q

Promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pMIR-Report-RASA1-3'UTR (mut - miR-206 site)

Plasmid

#62576

Purpose

Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Expression

Mutation

miR-206 binding site mutated (ACATTCCA --> AAC…

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pLY57_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2-TRAC-RHA(3166mut)

Plasmid

#192188

Purpose

CD22 CAR AAV vector PRODH2 KI (pLY057)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

CD22 CAR AAV vector PRODH2 KI (pLY057) (CD22 Synthetic)

Use

AAV; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-MUT-MRE

Plasmid

#235148

Purpose

Expresses the mCherry-based mutated (control) sensor for miR-124. TagBFP2 is expressed as a transduction reporter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mCherry with a mutated miR-124 MRE

Use

AAV

Tags

Expression

Mutation

7 nucleotides of the miR-124 WT MRE mutated

Promoter

EFS

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A)

Plasmid

#226577

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

I178A, L182A, L186A, V190A

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)

Plasmid

#206464

Purpose

Oskar 139-240 mut expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-240 mut (osk Fly)

Use

Tags

PH mcherry

Expression

Insect

Mutation

A162E L228E)

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A_S

Plasmid

#147515

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A (gw Human, Nematode)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A_R

Plasmid

#147389

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A (gw Fly, Nematode)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge