We narrowed to 1,197 results for: Aspa

1. pET29a(+)::D1141A

   Plasmid

   #192963

   Purpose

   Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   Tse5_D1141A
   

   Use

   Tags

   9xhis + TEV tag

   Expression

   Bacterial

   Mutation

   D1141A

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNB205

   Plasmid

   #169116

   Purpose

   TESEC expression vector for Asd from M. tuberculosis

   Depositor

   Edwin Wintermute

   Article

   Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

   Insert

   asd (asd Mycobacterium tuberculosis H37Rv)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pBAD

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AlkB-D135G

   Plasmid

   #160434

   Purpose

   A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

   Depositor

   Tao Pan

   Article

   Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

   Insert

   E. coli AlkB
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 135 to Glycine; deleted the…

   Promoter

   T7

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FoxFTVC+bpFOG_MEK-S216D, S220E

   Plasmid

   #107522

   Purpose

   Constitutive activation of FGF/MAPK pathway by MEK-S216D, S220E mutant

   Depositor

   Lionel Christiaen

   Article

   Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

   Insert

   MEK
   

   Use

   Tags

   Expression

   Mutation

   changed Serine 216 to Aspartic Acid and Serine 22…

   Promoter

   TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Thy1.1-muSOX D219A

   Plasmid

   #131704

   Purpose

   encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

   Depositor

   Britt Glaunsinger

   Article

   Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

   Insert

   Thy1.1-muSOX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Aspartic acid 219 mutated to an alanine

   Promoter

   CMV

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ORF18(G145A_N146A)-3xFLAG pCDNA4

   Plasmid

   #129812

   Purpose

   Expresses KSHV ORF18 (G145A_N146A) with Cterm 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 145 to Alanine and Asparagine 146…

   Promoter

   CMV

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. ORF18(N63A)-3xFLAG pCDNA4

   Plasmid

   #129780

   Purpose

   Expresses KSHV ORF18 (N63A) with Cterm 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Asparagine 63 to Alanine

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ORF18(N146A)-3xFLAG pCDNA4

   Plasmid

   #129798

   Purpose

   Expresses KSHV ORF18 (N146A) with Cterm 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Asparagine 146 to Alanine

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTJK513

   Plasmid

   #121470

   Purpose

   CEN LEU2 CNB1mutEF1

   Depositor

   Tami Kingsbury

   Article

   Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

   Insert

   CNB1mutEF1 (CNB1 Budding Yeast)
   

   Use

   Yeast cen plasmid

   Tags

   Expression

   Mutation

   Changed aspartic acid 34 to alanine

   Promoter

   Endogenous genomic promoter region

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTJK511

    Plasmid

    #121468

    Purpose

    2µm HIS3 CNB1mutEF4

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF4 (CNB1 Budding Yeast)
    

    Use

    Yeast high copy 2 micron vector

    Tags

    Expression

    Mutation

    Changed aspartic acid 144 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTJK510

    Plasmid

    #121465

    Purpose

    2µm HIS3 CNB1mutEF3

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF3 (CNB1 Budding Yeast)
    

    Use

    Yeast high copy 2 micron vector

    Tags

    Expression

    Mutation

    Changed aspartic acid 103 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTJK509

    Plasmid

    #121464

    Purpose

    2µm HIS3 CNB1mutEF2

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF2 (CNB1 Budding Yeast)
    

    Use

    Yeast high copy 2 micron vector

    Tags

    Expression

    Mutation

    Changed aspartic acid 66 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTJK508

    Plasmid

    #121463

    Purpose

    2µm HIS3 CNB1mutEF1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF1 (CNB1 Budding Yeast)
    

    Use

    Yeast high copy 2 micron vector

    Tags

    Expression

    Mutation

    Changed aspartic acid 34 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTJK516

    Plasmid

    #121473

    Purpose

    CEN LEU2 CNB1mutEF4

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF4 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 144 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTJK506

    Plasmid

    #121461

    Purpose

    CEN HIS3 CNB1mutEF4

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF4 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 144 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTJK505

    Plasmid

    #121460

    Purpose

    CEN HIS3 CNB1mutEF3

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF2 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 103 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTJK504

    Plasmid

    #121459

    Purpose

    CEN HIS3 CNB1mutEF2

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF2 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 66 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTJK503

    Plasmid

    #121458

    Purpose

    CEN HIS3 CNB1mutEF1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1mutEF1 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Tags

    Expression

    Mutation

    Changed aspartic acid 34 to alanine

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBDU-Atg17(F317D, I325A)

    Plasmid

    #122275

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid; Isoleucine 32…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGAD-Atg17(F317D, I325A)

    Plasmid

    #122273

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid; Isoleucine 32…

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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