We narrowed to 58,902 results for: SAP

1. Kv7.5/pcDNA3.1

   Plasmid

   #111498

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SR140

   Plasmid

   #156037

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SR140 (U2SURP Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28b-FOXP2_P6

   Plasmid

   #122649

   Purpose

   Expresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cells

   Depositor

   Hendrik Dietz

   Article

   Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

   Insert

   Homo sapiens forkhead box P2 (FOXP2 Human)
   

   Use

   Tags

   His-tag and Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGM32DEST

   Plasmid

   #74747

   Purpose

   Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

   Depositor

   Jordan Ward, Keith Yamamoto

   Article

   Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

   Inserts

   Gateway cassette
   QF-GR (NR3C1 Neurospora crassa, Synthetic, Human)
   

   Use

   Tags

   Fusion of the Neurospora crassa QF activator to t…

   Expression

   Worm

   Mutation

   Promoter

   No promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-neo-hApg5-HA

   Plasmid

   #22948

   Depositor

   Noboru Mizushima

   Article

   Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

   Insert

   Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-eGFP-dSaCas9-blast

   Plasmid

   #191653

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   eGFP-T2A-dSaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNATM3.1/V5-His TOPO-PKCi-K274W

   Plasmid

   #55690

   Purpose

   expresses dominant negative human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E and K274W

   Promoter

   CMV

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

9. THOC5

   Plasmid

   #156084

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   THOC5 (THOC5 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNATM3.1/V5-His TOPO-PKCi-A120E

    Plasmid

    #55687

    Purpose

    expresses constitutively active human PKCi tagged with V5

    Depositor

    Pedro Salas

    Article

    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

    Insert

    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    A120E

    Promoter

    CMV

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pAS4.1w-tet-on-NORAD

    Plasmid

    #125010

    Purpose

    express in mammalian cell

    Depositor

    Ruey-Hwa Chen

    Article

    Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

    Insert

    Homo sapiens non-coding RNA activated by DNA damage (NORAD), long non-coding RNA (NORAD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-Tight

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. THOC6

    Plasmid

    #156085

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    THOC6 (THOC6 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SF3B3-1

    Plasmid

    #156004

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SF3B3 (SF3B3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SF3B3

    Plasmid

    #156003

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SF3B3 (SF3B3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    S6L

    Promoter

    EF-1a

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RBM22

    Plasmid

    #155889

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    RBM22 (RBM22 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJ414-PCY1:S495A

    Plasmid

    #98151

    Purpose

    Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:S495A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed serine 495 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ414-PCY1:W603A

    Plasmid

    #98153

    Purpose

    Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:W603A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tryptophan 603 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJ414-PCY1:H695Q

    Plasmid

    #98154

    Purpose

    Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:H695Q
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed histidine 695 to glutamine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJ414-PCY1:H695A

    Plasmid

    #98155

    Purpose

    Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:H695A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed histidine 695 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJ414-PCY1:Y696G

    Plasmid

    #98156

    Purpose

    Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:Y696G
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tyrosine 696 to glycine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge