Addgene

Showing: 861 - 880 of 1667 results

Results per page:

Sort results by:

p3HD1RLuc2

Plasmid

#236276

Purpose

Mammalian expression of human dopamine D1 receptor; N-terminal HA tag, C-terminal Rluc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

dopamine D1 receptor (DRD1 Human)

Use

Tags

3xHA and Rluc8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p3HM1Luc4

Plasmid

#236277

Purpose

Mammalian expression of human muscarinic M1 receptor; N-terminal HA tag, C-terminal Rluc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

muscarinic M1 receptor (CHRM1 Human)

Use

Tags

3xHA and Rluc8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pToxAmp21R

Plasmid

#218287

Purpose

A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pToxAmp12

Plasmid

#218286

Purpose

Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pToxAmp1

Plasmid

#218285

Purpose

Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GyrA_F164R238

Plasmid

#216230

Purpose

S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Changed arginine 238 to serine, phenylalanine 168…

Promoter

T7

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GyrB_A467

Plasmid

#216234

Purpose

S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Changed alanine 467 to valine

Promoter

T7

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GyrB_T522

Plasmid

#216235

Purpose

S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Changed threonine 522 to isoleucine

Promoter

T7

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mScarlet-I-eDHFR-ORP5(ΔPH)

Plasmid

#214277

Purpose

Mammalian expression of ORP5(ΔPH) fused to mScarlet-I-tagged E. coli DHFR

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mScarlet-I-eDHFR-ORP5(253-879) (OSBPL5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsSmg6-sh(t10)RNAres_S

Plasmid

#147460

Purpose

Mammalian Expression of HsSmg6-sh(10)RNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg6-sh(10)RNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsSmg7-siRNAres-shRNAres_S

Plasmid

#147463

Purpose

Mammalian Expression of HsSmg7-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres-shRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg7-G100E-siRNAres-V5-SBP-MBP_S

Plasmid

#147472

Purpose

Mammalian Expression of HsSmg7-G100E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-G100E-siRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg7-K66ER163E-siRNAres-V5-SBP-MBP_S

Plasmid

#147473

Purpose

Mammalian Expression of HsSmg7-K66ER163E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-K66ER163E-siRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg7-siRNAres-V5-SBP-MBP_S

Plasmid

#147474

Purpose

Mammalian Expression of HsSmg7-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-HsUpf1_115-1129-S1107A-siRNAres_S

Plasmid

#147477

Purpose

Mammalian Expression of HsUpf1_115-1129-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1_115-1129-S1107A-siRNAres (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

4 non silent mutations E167G, K282R, A442G and T1…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-HsUpf1-S1107A-siRNAres_S

Plasmid

#147478

Purpose

Mammalian Expression of HsUpf1-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1-S1107A-siRNAres (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

5 non silent mutations A69S, E167G, K282R, A442G…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg5-G120E-siRNAres-V5-SBP-MBP_S

Plasmid

#147470

Purpose

Mammalian Expression of HsSmg5-G120E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg5-G120E-siRNAres (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet1-His-TEV-OTC_d1_Ub

Plasmid

#170729

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The C-terminus of OTC was truncated by removing o…

Promoter

T7

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-SPA mimic WT

Plasmid

#92350

Purpose

To mimic SPA RNA processing and expression

Depositor

Ling-Ling Chen

Article

Wu et al Mol Cell. 2016 Nov 3;64(3):534-548. doi:

Insert

SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge