We narrowed to 5,482 results for: pcas
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Plasmid#127908PurposeWT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta geneDepositorInsertgRNA for Human 5' HP1beta
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAT.334
Plasmid#119556PurposeLevel T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2963_eSpCas9-PGKHygdtkCh
Plasmid#124205PurposeExpression of eSpCas9(1.1) and sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9-J23113
Plasmid#119701PurposeExpression of Cas9 with a weak promoterDepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT3-FKBP_SL
Plasmid#107302PurposeExpresses R1335K mutant SpCas9 fused to FKBP in mammalian cellsDepositorInsertSpCas9
UseCRISPRTagsFKBPExpressionMammalianMutationR1335K and fused to FKBPPromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_m-AKAR5
Plasmid#114139PurposeMammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 C-terminally tagged by KRas C tail
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cyt-AKAR5
Plasmid#114138PurposeMammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 with C-terminal nuclear export signal (NES)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::zwf_fbp_opcA
Plasmid#101846PurposeReplaces native promoter of zwf_fbp_opcA with Ptrc promoterDepositorInsertsp/st resistance casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW751_pCAG-PV5
Plasmid#87565PurposeSimple PV5DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW752_pCAG-PV6
Plasmid#87566PurposeSimple PV6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW755_pCAG-pDest-U5-UX
Plasmid#89507PurposepCAG destination vector (U5-UX)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW753_pCAG-PV7
Plasmid#87567PurposeSimple PV7DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW754_pCAG-PV8
Plasmid#87568PurposeSimple PV8DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gapAP1
Plasmid#87146PurposegapAP1 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgapAP1 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch5_155183064-155183141
Plasmid#81211PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HDAC4*
Plasmid#71402PurposeCAG promoter, mammalian expressionDepositorInsertHDAC4* (HDAC4 Human)
UseTagsExpressionMammalianMutationAfter aa126, insertion leads to reading frame shi…PromoterCAGAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-zwf
Plasmid#65825Purposezwf targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertzwf guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA
Plasmid#65818PurposeudhA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertudhA guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-proD
Plasmid#65820PurposeConst. Promoter ProD targetting RNA E. coli Low Phosphate InductionDepositorInsertproD guide
UseTagsExpressionMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2
Plasmid#65817PurposegltA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA2 guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only