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We narrowed to 14,379 results for: TIM

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Showing: 8801 - 8820 of 14379 results

  7. pAAV uN2CpolyGly op GFP (NIID)

    Plasmid
    #224356
    Purpose
    Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)
    Depositor
    Nicolas Charlet-Berguerand
    Article
    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.
    Insert
    uN2C with a GGC repeat expansion (100x)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Codon-optimized for expression in human, so no pu…
    Promoter
    CMV + chimeric intron (CAG)
    Available Since
    Feb. 5, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224356-plasmid-map-sequence-id-460492

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  8. PSB-CMV-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250913
    Purpose
    Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS1-2 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (SEC61B, ARFGAP1 Human)
    Tags
    EGFP on Sec61b and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available Since
    Feb. 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    250913-plasmid-map-sequence-id-498650

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  9. PSB-CMV-Puro ArfGAP1_ALPS1-mkate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250912
    Purpose
    Dual color expression of mKate2-3NLS tagged rat ARFGAP1 ALPS1 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (Arfgap1, SEC61B Rat, Human)
    Tags
    EGFP on Sec61B and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 196–234 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250912-plasmid-map-sequence-id-498488

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  11. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107942-plasmid-map-sequence-id-259760

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  14. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

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  15. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  16. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128096-plasmid-map-sequence-id-249669

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  17. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114072-plasmid-map-sequence-id-220522

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Showing: 8801 - 8820 of 14379 results