We narrowed to 25,289 results for: promoter
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Plasmid#100016PurposeTo over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.DepositorInsertInsulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
UseLentiviralExpressionMammalianPromoterSPTA1 erythroid specific promoterAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)
Plasmid#208959PurposeA variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
actc1b-GcAMP5
Plasmid#126563PurposeMuscle specific expression of the calcium reporter GcAMP5 in zebrafish. Parton lab clone APTDepositorInsertGcAMP5
Available SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-2xMyc-Rac1-WT
Plasmid#128580PurposeFor mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.DepositorAvailable SinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)
Plasmid#208954PurposeA variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A), Phi29(-exo;D169A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pIII pm
Plasmid#218100PurposePhagemid that expresses LacI, and pIII under the Lac promoterDepositorInsertpIII
ExpressionBacterialPromoterLac promoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197024PurposeVector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized OsCas12f1
ExpressionMammalianPromoterCBh, CMV, hU6Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanCOL2A1enhancer
Plasmid#173961PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer region from COL2A1 gene
UseLuciferaseMutationNonePromoterSV40 promoterAvailable SinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF-sh-hBVRA
Plasmid#100285PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for human BVRA
TagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cas12Max
Plasmid#195334Purposevector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized Cas12Max
Tags3xFlagExpressionMammalianMutationN243RPromoterCBh, CMV, hU6Available SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-HsBIN1_tv8
Plasmid#176012PurposeMammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBHDepositorInsertBin1
TagsEGFPExpressionMammalianAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Nr4a1 double gRNAs
Plasmid#162791PurposeMultiplex Guide RNAs to generate Nr4a1 knockout by CRISPRDepositorAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG_FBXL7
Plasmid#171848PurposepcDNA3-FLAG_FBXL7DepositorAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
LRG/Foxa1 e2.4
Plasmid#105511PurposeLentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNADepositorInsertFoxa1 (sgRNA, e2.4)
UseLentiviralAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1aGFP2AKAT7-W
Plasmid#159292PurposeLentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoterDepositorInsertKAT7 (KAT7 Human)
UseLentiviralExpressionMammalianMutationCRISPR sites mutated (gRNA ID. 5 and A10)Promoterhuman EF1a promoterAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pIV pm
Plasmid#218101PurposePhagemid that expresses LacI, and pIV under the Lac promoterDepositorInsertpIV
ExpressionBacterialPromoterLac promoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3 1 kb prom. + Enhancer CD274
Plasmid#107005PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianMutationN.B. There are two published SNPs in this region …Available SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final
Plasmid#107009PurposeModified Luciferase expression vectorDepositorAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCELF4-4405
Plasmid#115466PurposeConstitutive lentiviral expressionDepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMVtight-HNF1A-FLAG-Hygro
Plasmid#183232PurposeLentiviral vector; Tet-on advanced system driving the expression of tagged HNF1ADepositorAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only