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CL20_mEGFP_TMPRSS2_ETV4

Plasmid

#205945

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ETV4 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ETV4 (TMPRSS2, ETV4 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TMPRSS2_ETV1

Plasmid

#205944

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ETV1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ETV1 (TMPRSS2, ETV1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS2-Spike-HiBiT

Plasmid

#191212

Purpose

Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SARS-CoV-2 spike_WT (S SARS CoV-2)

Tags

HiBiT (high affinity small nanoluciferase fragmen…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (EG.5)

Plasmid

#241114

Purpose

Expresses SARS-CoV-2 spike (EG.5) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (EG.5) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I, L24del, P25del, P26del, A27S, V83A, G142D, …

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_XBB

Plasmid

#194494

Purpose

expressing SARS-CoV-2 XBB spike protein for pseudovirus production

Depositor

David Nemazee

Article

Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

Insert

Spike (XBB) (S SARS-CoV-2)

Expression

Mammalian

Mutation

V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

Promoter

CMV

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

Plasmid

#179905

Purpose

Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

Insert

SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BQ.1.1

Plasmid

#194493

Purpose

expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

Insert

Spike (BQ.1.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T, K444T, N460K and c-terminal 18aa del on th…

Promoter

CMV

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_FURIN_WT

Plasmid

#82122

Purpose

Gateway Donor vector containing FURIN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FURIN (FURIN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLD-puro-Cc-SARS-CoV-2_NSP16-VA

Plasmid

#191215

Purpose

Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

Non structural protein 16 (ORF1ab SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BA.4/5

Plasmid

#186810

Purpose

pseudovirus plasmid expressing BA.4 and BA.5 spike protein

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

43740568 (S SARS-CoV-2)

Expression

Mammalian

Mutation

HY69-70del, L452R, F486V, Q493R, on the basis of …

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

Plasmid

#169191

Purpose

To express nsp10-nsp16 fusion protein in E. coli

Depositor

John Diffley

Article

Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

Insert

14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-OMICRON.BA.2-RBD

Plasmid

#184830

Purpose

Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Article

Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

Insert

S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)

Tags

6X His tag

Expression

Mammalian

Mutation

SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pABVec-NbH11-D4_huIgG1

Plasmid

#160314

Purpose

Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusion

Depositor

Ray Owens

Article

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

VHH D4-Fc

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.3_SARS2_BA.2.12.1

Plasmid

#186809

Purpose

pseudovirus plasmid that expresses spike of BA.2.12.1

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2.12.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

L452Q, S704L on the basis of BA.2, c-terminal 18 …

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pABVec-NbH11-H4_huIgG1

Plasmid

#160313

Purpose

Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusion

Depositor

Ray Owens

Article

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

VHH H4-Fc

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pαH-S-GSAS-OMICRON.BA.1-RBD

Plasmid

#184831

Purpose

Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Article

Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

Insert

S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)

Tags

6X His tag

Expression

Mammalian

Mutation

SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform2_Flag

Plasmid

#191999

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2 (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform1_untagged-Puro

Plasmid

#192001

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Spike Display_B.1.1.7

Plasmid

#172735

Purpose

Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S B.1.1.7 (S Severe acute respiratory syndrome coronavirus 2)

Tags

3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

Expression

Mammalian

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Promoter

CMV

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Spike Display_B.1.1.248

Plasmid

#172737

Purpose

Expression vector for SARS-CoV-2 (B.1.1.248) spike used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S B.1.1.248 (S Severe acute respiratory syndrome coronavirus 2)

Tags

3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

Expression

Mammalian

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Promoter

CMV

Available Since

Aug. 18, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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