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PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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MPRA_minCMV+GFP

Plasmid

#204866

Purpose

Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.

Depositor

Philipp Maass

Article

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Insert

minCMV:GFP

Expression

Bacterial and Mammalian

Promoter

minimal CMV

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

Plasmid

#227246

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

Depositor

Jae Seong Lee

Article

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

EF1α and pTRE3G

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCXWB-EBNA1

Plasmid

#37624

Purpose

Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Mm.cargo(Cas9)

Plasmid

#174865

Purpose

Expresses mouse Peg10 cargoRNA encoding SpCas9

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

SpCas9 flanked by MmPeg10 UTRs

Tags

HA-SV40 NLS and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(WT)-V5

Plasmid

#158663

Purpose

Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(WT) AAVS1

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMVmin-luc2

Plasmid

#194384

Purpose

Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

6xCRE-CMVmin-luc2

Expression

Mammalian

Promoter

CMV minimal

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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DUSP6

Plasmid

#40772

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

DUSP6 (DUSP6 Human)

Tags

6His and TEV

Expression

Bacterial

Mutation

Starting Met removed

Promoter

T7

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pAAV/L7-6-GFP-WPRE

Plasmid

#126462

Purpose

An AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.

Depositor

Hirokazu Hirai

Article

Nitta et al Mol Ther Methods Clin Dev. 2017 Jul 27;6

Insert

L7-6, Purkinje cell specific promoter

Use

AAV

Expression

Mammalian

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-RFC1

Plasmid

#59209

Purpose

Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Lentiviral; Damid

Tags

Dam and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-VA

Plasmid

#220893

Purpose

Express haA3A-CBE-VA (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBJ5 hBRG1

Plasmid

#17873

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg (SMARCA4 Human)

Expression

Mammalian

Mutation

R1608Q

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pBJ5 BRG1 DN

Plasmid

#17874

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg DN (SMARCA4 Human)

Expression

Mammalian

Mutation

dominant negative

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pcpBpaRS_v2

Plasmid

#196485

Purpose

To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

Depositor

Kensaku Sakamoto

Article

Hino et al Methods Mol Biol. 2012;794:215-28. doi:

Insert

benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA

Expression

Mammalian

Promoter

SV40(synthetase), Pol III tRNA promoter (tRNA), C…

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcpAzpaRS_v2

Plasmid

#196483

Purpose

To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

Depositor

Kensaku Sakamoto

Article

Hino et al Methods Mol Biol. 2012;794:215-28. doi:

Insert

azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA

Expression

Mammalian

Promoter

SV40(synthetase), Pol III tRNA promoter (tRNA), C…

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A-nCas9

Plasmid

#163535

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSFV_eGFP-P2A-luc2pre (3'UTR)_RLEloop

Plasmid

#240255

Purpose

Attenuated PROTEUS vector with eGFP-LUC transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

EGFP-P2A-firefly luciferase

Expression

Mammalian

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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FLAG-MED8

Plasmid

#15406

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

MED8 (MED8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only