We narrowed to 9,980 results for: Uty
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Plasmid#225619PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHis and mCherry-V5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-375_Cacnes_phlF
Plasmid#225620PurposeC. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.DepositorInsertPhlF
UseSynthetic BiologyTags6xHis and mCherry-V5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human CSF-1R-Y969F,S301-IRES—GFP
Plasmid#115524PurposeMammalian expression constructDepositorInserthuman CSF-1 receptor constitutively activated mutant (CSF1R Human)
UseRetroviralMutationY969F,S301Available SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B15
Plasmid#135513PurposeMammalian expression of myc-tagged HLA-B*15:01DepositorInsertHLA-B*15:01 (HLA-B Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
nLYN267-gs10-NLS-VPR
Plasmid#188265PurposePlasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnLYN267 (LYN Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
TA1-DuET
Plasmid#213382PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102887PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PdTK-CAG-mCh-[uBglII]
Plasmid#107280Purposepuro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherryDepositorInsertAAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
Available SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102888PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
RXFP1-DuET
Plasmid#213373PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
nERb446-gs10-NLS-VPR
Plasmid#188263PurposePlasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnERb446 (ESR1 Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
FPR3-DuET
Plasmid#213241PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shQKI-3371
Plasmid#115455PurposeConstitutive lentiviral expressionDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR-U6gRNA-PGKpuro2APDGFB
Plasmid#136409PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.DepositorTypeEmpty backboneUseCRISPRMutationBbsI restriction site in the PDGFB sequence (Glu1…PromoterU6, PDKAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM671 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 in TUPV3
Plasmid#161573PurposeConstitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 under the EF1α promoterDepositorInsertTC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterEF1αAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald
Plasmid#203803PurposeLentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-WT
Plasmid#82030PurposePlasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
TagsHAExpressionMammalianMutationMutations making the cDNA resistant to a siRNAPromoterCMV-tetAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemExpressionMammalianMutationY64L (mutRFP)Available SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-HA-PIK3CA(H1047R;I848V)
Plasmid#33253DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and isoleucine…PromoterSV40Available SinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only