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pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_1-560 USP7

Plasmid

#131244

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 1 - 560. This truncati…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_1-205 USP7

Plasmid

#131245

Purpose

Mammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses a mutant form of our pcDNA3.1-N-Myc_1-5…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF1-PTPRD-G446E

Plasmid

#25643

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta (PTPRD Human)

Use

Lentiviral

Expression

Mammalian

Mutation

tumor-derived mutation created by site-directed m…

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
hs-lambda btl

Plasmid

#17581

Depositor

Denise Montell

Article

Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

Insert

lambda-btl (btl Fly)

Expression

Insect

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD

Plasmid

#14442

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 CTD (BRD4 Human)

Tags

SV40 NLS

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD). Function…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
CRISPRmTmG2

Plasmid

#69992

Purpose

The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA that targets near LoxP sites

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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BmTyr-Flag

Plasmid

#220779

Purpose

non-targeted BmTyr throughout the cytoplasm

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

Bacillus megaterium Tyrosinase

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pL2-G

Plasmid

#137090

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pICE TRP1

Plasmid

#122881

Purpose

TRP1 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pICE LYS2

Plasmid

#122879

Purpose

LYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-KanMx

Plasmid

#122923

Purpose

TRP1-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-NatMx

Plasmid

#122924

Purpose

TRP1-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pQcre1

Plasmid

#135659

Purpose

Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

ppag promoter

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HIS3

Plasmid

#122912

Purpose

TRP1-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-LEU2

Plasmid

#122905

Purpose

ADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HphMx

Plasmid

#122922

Purpose

TRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

July 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMKV001

Plasmid

#35107

Depositor

John McCusker

Article

Vorachek-Warren et al Yeast. 2004 Jan 30;21(2):163-71.

Insert

dsdaMX4

Use

Yeast replicating

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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lvl0 HSPt

Plasmid

#153378

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

HSP terminator

Expression

Plant

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 NDUFA8t

Plasmid

#153379

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

NDUFA8 terminator

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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