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plko.1/shMCT4 477

Plasmid

#99598

Purpose

Knockdown of MCT4 expression

Depositor

Eli Bar

Article

Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

Insert

MCT4 (SLC16A3 Human)

Use

Lentiviral

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(−) mCER-Prx2

Plasmid

#158224

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Short P2A

Plasmid

#164116

Purpose

Detect the short Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Short P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TCF21

Plasmid

#192894

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TCF21

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TCF21 (TCF21 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

Plasmid

#107942

Purpose

Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)

Tags

3x HA and NLS (nucleoplasmin)

Expression

Mammalian

Mutation

E174R, S542R, K548R, and N282A

Promoter

CAG

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-aSYNUCLEIN A53T

Plasmid

#227004

Purpose

AAV transfer plasmid encoding the human A53T mutant α-SYN under the control of the CMVie enhanced synapsin1 promoter

Depositor

Veerle Baekelandt

Article

Van der Perren et al J Vis Exp. 2016 Feb 28;(108):53670. doi:

Insert

alpha synuclein A53T mutant (SNCA Human, Synthetic)

Use

AAV

Mutation

A53T

Promoter

CMVenh synapsin

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-sRGECO

Plasmid

#137128

Purpose

Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSR646

Plasmid

#65215

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-6 cap

Use

AAV

Expression

Insect

Promoter

P10 and polyhedrin

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMK464 (mAB-Neo)

Plasmid

#214390

Purpose

mAID-BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

mAID-BromoTag-Neo

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF7:1-342

Plasmid

#211118

Purpose

Mammalian expression of full-length human DCAF7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF7:M1-V342 (DCAF7 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S33Y Beta-catenin

Plasmid

#19286

Purpose

Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S33Y (presumptive GSK3β phosphorylation site)

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA-R-diLACCO1

Plasmid

#208028

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-diLACCO1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pB-nCas9-PBE

Plasmid

#98160

Purpose

Expresses CRISPR base editor in maize

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-nCas9-UGI-NLS

Use

Maize

Expression

Plant

Promoter

Ubi

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-FLAG-mCherry-C1-centrin-1

Plasmid

#73333

Purpose

Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

Depositor

Manuel Thery

Insert

centrin-1 (CETN1 Human)

Use

Lentiviral

Tags

FLAG and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti PGK GFP Puro (w509-5)

Plasmid

#19070

Purpose

3rd gen lentiviral eGFP expression vector, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMJ920

Plasmid

#42234

Depositor

Jennifer Doudna

Article

Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

Insert

Cas9

Use

CRISPR

Tags

GFP, HA epitope, and NLS (nuclear localization si…

Expression

Mammalian

Mutation

codon-optimized synthetic DNA sequence

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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HIV7-Bcl2-CD28-CD3z-T2A-EGFR

Plasmid

#163465

Purpose

Lentiviral vector encoding a Bim-specific chimeric antigen receptor with a synthetic Bcl2 binding element

Depositor

David Baker

Article

Lajoie et al Science. 2020 Sep 25;369(6511):1637-1643

Insert

Bim-specific CAR

Use

Lentiviral

Tags

HA

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC12-pA

Plasmid

#220776

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

Insert

oScarlert-WPRE-BC12

Use

AAV

Promoter

CAG

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA-GW

Plasmid

#209333

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJWV102-PL-dCas9

Plasmid

#85588

Purpose

Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

dCas9sp

Use

CRISPR

Expression

Bacterial

Promoter

PLsp

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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