We narrowed to 9,972 results for: crispr plasmids

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pPD0039_CROP-seq-CAR-Puro

Plasmid

#242532

Purpose

CAR T screening

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CD19-BB CAR (CD19 Human)

Use

Lentiviral

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6d

Plasmid

#207854

Purpose

Mammalian expression of PE6d prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6d

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPD0035_CROPseq-CAR-Puro_CD19-28z

Plasmid

#242983

Purpose

CD19-28z FMC63 CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CD19-28z FMC63 CAR (CD19 Human)

Use

Lentiviral

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6a

Plasmid

#207851

Purpose

Mammalian expression of PE6a prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6a

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONOR-PARK6e5-I368N

Plasmid

#86154

Purpose

Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

PINK1 exon 5 homology arms (PINK1 Human)

Expression

Bacterial and Mammalian

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmaxRNaseHtrunc

Plasmid

#207858

Purpose

Mammalian expression of PEmaxDRNaseH prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PEmaxDRNaseH

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PB-GFP-Gal8

Plasmid

#127191

Purpose

Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Insert

GFP-Gal8 (LGALS8 Human)

Tags

Gal8 is fused to the c-terminus of GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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UBC-TLR4-HA hygro

Plasmid

#246905

Purpose

Expresses HA-tagged human TLR4 in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

TLR4 (TLR4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE N-terminal

Plasmid

#137175

Purpose

AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE N-terminal; U6-protospacer

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_NANOS3_cterm

Plasmid

#222904

Purpose

Cas9/sgRNA plasmid for targeting NANOS3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NANOS3 sgRNA (NANOS3 Synthetic, Human)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-FOXL2-cterm

Plasmid

#192893

Purpose

Expresses Cas9 and sgRNA targeting the C-terminus region of FOXL2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2 C-term sgRNA (FOXL2 Synthetic)

Use

CRISPR

Promoter

U6

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_TFAP2C_cterm

Plasmid

#222916

Purpose

Cas9/sgRNA plasmid for targeting TFAP2C

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, TFAP2C sgRNA (TFAP2C Synthetic, Human)

Use

CRISPR

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6e

Plasmid

#207855

Purpose

Mammalian expression of PE6e prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6e

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, K918A, K775R)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6g

Plasmid

#207857

Purpose

Mammalian expression of PE6g prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6g

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6f

Plasmid

#207856

Purpose

Mammalian expression of PE6f prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6f

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mAC-POLR2A donor (Hygro)

Plasmid

#124496

Purpose

A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)

Use

CRISPR

Tags

mAID-mClover

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-NPM1-repair-template for U2OS

Plasmid

#238246

Purpose

For knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-NPM1-gRNA for U2OS

Plasmid

#238250

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_051_dCas9-KRAB-HA

Plasmid

#228936

Purpose

All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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