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pAM4937 (pCV0049)

Plasmid

#120082

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Blasto

Plasmid

#167864

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRS306-pHIS3-2xGFP-tURA3

Plasmid

#116936

Purpose

Yeast integrative vector for the expression of 2xGFP

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

2xGFP

Expression

Yeast

Promoter

HIS3

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_INT

Plasmid

#68436

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-mir124a

Plasmid

#61539

Purpose

Expresses mir124a in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

mir124a (MIR124-1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
p6614 MSCV-IP N-HAonly PXMP3

Plasmid

#35012

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PXMP3 (PEX2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pJH3626

Plasmid

#179630

Purpose

Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

RFP

Expression

Worm

Promoter

Pacr-2(s)

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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HS-OS-Plk4-mKate2

Plasmid

#105944

Purpose

heat shock inducible transgenesis, non phoshorylable version of Plk4

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Plk4 (Plk4 Mouse)

Tags

mKate2

Expression

Bacterial

Mutation

1002 to 3704 of Plk4/ deletion of S282-S305.

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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mADAM17deltaMP in pQCXIP cFLAG

Plasmid

#19144

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM17 (Adam17 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 189-378

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pT7-HISx6-SIMx6

Plasmid

#116938

Purpose

Bacterial expression vector for 6 sim

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

6 sim

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SUMOx10

Plasmid

#116937

Purpose

Bacterial expression vector for 10 sumo

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10 SUMO

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:GLuc:bgH

Plasmid

#135294

Purpose

Transcriptional unit for constitutive expression of GLuc in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:GLuc:bGH

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_mcherry

Plasmid

#167886

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
pSAG1::CAS9-U6::sg290860-6

Plasmid

#59855

Purpose

Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

Depositor

David Sibley

Article

Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

Insert

CRISPR sg290860-6

Use

CRISPR; ; toxoplasma gondii

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pJH4542

Plasmid

#200779

Purpose

Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GCaMP6

Tags

mNeptune

Expression

Worm

Promoter

Ptwk-40

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-TRIM24-C840W

Plasmid

#28136

Depositor

Michelle Barton

Article

Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V and C840W

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5

Plasmid

#60532

Purpose

Donor vector for human Pak5

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway Cloning

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_TLOC1 (399aa)

Plasmid

#45288

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-lxlplap

Plasmid

#45581

Depositor

Nirao Shah

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Inserts

Green fluorescent protein
Placental alkaline phosphatase (ALPP Human)

Use

Lentiviral

Tags

Histone 2B

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pJH3628

Plasmid

#191445

Purpose

Prgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural expression of Chrimson RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Chrimson

Tags

wCherry

Expression

Worm

Promoter

Pregf-1

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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