We narrowed to 951 results for: kbp

1. pX330-H11r1-2-DD-Cas9

   Plasmid

   #85978

   Purpose

   Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUASt-ssBiP-ESCargo

   Plasmid

   #140166

   Purpose

   Expresses a regulatable secretory cargo for Drosophila melanogaster

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hs70

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBetaActin-dCer3-C2-FRB

   Plasmid

   #191327

   Purpose

   Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FRB-C2-dCer3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBetaActin-FRB-dCer3-CAAX

   Plasmid

   #191326

   Purpose

   Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FRB-dCer-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. iXFGFR1k

   Plasmid

   #31259

   Depositor

   Masahiro Yamagishi

   Article

   Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

   Insert

   Xenopus FGFR1-ICD-FKBP (fgfr1-a Frog)
   

   Use

   Tags

   HA epitope tag and myristoylation signal

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-FRB-VMAc-CGFP

   Plasmid

   #70226

   Purpose

   Expresses C-terminal portion of GFP along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   eGFP
   

   Use

   Tags

   FKBP-rapamycin binding (FRB) domain of the mammal…

   Expression

   Mammalian

   Mutation

   Changed Ile 129 to Cys, Deleted amino acids 1-128

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-FRB-VMAc-CSarcin

   Plasmid

   #70223

   Purpose

   Expresses VMA intein with C-terminal portion of alpha-sarcin

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (C-terminal portion)
   

   Use

   Tags

   FKBP-rapamycin binding (FRB) domain of the mammal…

   Expression

   Mammalian

   Mutation

   Changed Asp 28 to Cys, deleted amino acids 1-27

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pEF-pIgH-ESCargo(FTV)

   Plasmid

   #140160

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. YF-RhoA(CA)

   Plasmid

   #20153

   Depositor

   Tobias Meyer

   Article

   Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

   Insert

   YFP-FKBP-RhoA(CA) (RHOA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF-pIgH-ESCargo(FTV,Crimson)

    Plasmid

    #140162

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. YF-Rac1(CA)

    Plasmid

    #20150

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Rac1(CA) (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    constitutively active mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-pIgH-ESCargo*

    Plasmid

    #140159

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-RapR-FAK-YM

    Plasmid

    #25928

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

    Insert

    RapR-FAK (Ptk2 Human, Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This pEGFP-RapR-FAK-YM plasmid contains the FAK m…

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. YF-Rac1(DN)

    Plasmid

    #20151

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Rac1(DN) (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dominant negative mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJin-290

    Plasmid

    #125718

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-FRB-HA no-pA

    Plasmid

    #82594

    Purpose

    3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRB-HA (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUASt-ssBiP-ESCargo*

    Plasmid

    #140165

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hs70

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAGH51

    Plasmid

    #107255

    Purpose

    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    HU-FRB
    

    Use

    Synthetic Biology

    Tags

    FRB

    Expression

    Mutation

    Promoter

    native HU promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX-EF1a_NKX2-1_CdTag-HA

    Plasmid

    #221493

    Purpose

    NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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