We narrowed to 26,202 results for: STI

1. pET16b-His-ArgRS

   Plasmid

   #124104

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Argin+I4 ligase (argS Escherichia coli)
   

   Use

   Tags

   10x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE30-His-AsnRS

   Plasmid

   #124105

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Asparaginyl-tRNA ligase (asnS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21a-MetRS-His

   Plasmid

   #124115

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Methionine--tRNA ligase (metG Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQE30-His-ThrRS

   Plasmid

   #124119

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Threonyl-tRNA ligase (thrS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21a-His-IleRS

   Plasmid

   #124112

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Isoleucyl-tRNA ligase (ileS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a-LeuRS-His

   Plasmid

   #124113

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Leucyl-tRNA ligase (leuS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21a-TrpRS-His

   Plasmid

   #124120

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Tryptophanyl-tRNA ligase (trpS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQE60-EFTu-His

   Plasmid

   #124127

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Elongation factor Tu (tufB Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a-CysRS-His

   Plasmid

   #124107

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Cysteinyl-tRNA ligase (cysS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a-MTF-His

    Plasmid

    #124133

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionyl-tRNA formyltransferase (fmt Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDonor14-ShHELIX-KanR (CJT70)

    Plasmid

    #181793

    Purpose

    pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQE30-His-IF2

    Plasmid

    #124124

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Initiation factor 2 (infB Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSR33.4r

    Plasmid

    #63198

    Purpose

    Expresses Cph8, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    Cph8
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQE30-His-NDK

    Plasmid

    #124136

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Nucleotide diphosphate kinase (ndk Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST-CMV-polysite

    Plasmid

    #122843

    Purpose

    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE30-His-IF1

    Plasmid

    #124123

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Initiation factor 1 (infA Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE30-His-IF3

    Plasmid

    #124125

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Initiation factor 3 (infC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQE30-His-RF3

    Plasmid

    #124131

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Release factor 3 (prfC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE60-EFTs-His

    Plasmid

    #124128

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor Ts (tsf Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b-His-RF2

    Plasmid

    #124130

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Release factor 2 (prfB Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pQE60-RRF-His

    Plasmid

    #124132

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Ribosome recycling factor (frr Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2-Gal4ff.zf1

    Plasmid

    #61392

    Purpose

    mRNA synthesis of Gal4ff codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Gal4ff.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRN114

    Plasmid

    #127221

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, Wus, AtSTM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGH8 - pRAB-3::mCherry::unc-54utr

    Plasmid

    #19359

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Prab-3::mCherry
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. M-PKD2 (OF2-3)

    Plasmid

    #21370

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type II (PKD2 Human)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    wild-type*

    Promoter

    Available since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCYP64

    Plasmid

    #212897

    Purpose

    Expresses the Priestia megaterium LRD-modifying cytochrome P450 enzyme

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Insert

    cytochrome P450
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…

    Promoter

    PcymRC

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJT119b

    Plasmid

    #50551

    Purpose

    Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Inserts

    CcaS
    CcaR
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMU-PLAr

    Plasmid

    #61200

    Purpose

    Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtRubisco1sp-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSEM371 - ce-unc-119 array integration

    Plasmid

    #182640

    Purpose

    Co-injection fragment for extra-chromosomal array integration at ce-unc-119 locus

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pETZZtevPP7CP(C68A,C73A)

    Plasmid

    #27548

    Depositor

    Kathleen Collins

    Article

    Hogg et al RNA. 2007 Jun . 13(6):868-80.

    Insert

    Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
    

    Use

    Tags

    TEV protease cleavage site, six histidine tag, an…

    Expression

    Bacterial

    Mutation

    changed two cysteine to alanine (C68A,C73A) to in…

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-CamKIIa-ChrimsonR::FusionRed::Kv2.1

    Plasmid

    #102770

    Purpose

    "Activate neurons with 2p stimulation"

    Depositor

    Karel Svoboda

    Article

    Daie et al Nat Neurosci. 2021 Feb;24(2):259-265. do

    Insert

    ChrimsonR::FusionRed::Kv2.1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1 traficking domain

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMM117

    Plasmid

    #127212

    Purpose

    BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AICSDP-117:POLR2A-mEGFP

    Plasmid

    #164500

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

    Depositor

    Allen Institute for Cell Science

    Insert

    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pC-GoldyTALEN

    Plasmid

    #38143

    Purpose

    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

    Depositor

    Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Tags

    AcV5 and FokI homodimer

    Expression

    Mammalian

    Mutation

    Truncate at N and C terminous

    Promoter

    miniCaggs

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

    Plasmid

    #19326

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::twk-18(cn110)cDNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FLS-PC1

    Plasmid

    #41520

    Depositor

    Thomas Weimbs

    Article

    Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    Myc/His

    Expression

    Mammalian

    Mutation

    sequence from 4107 - 4303 of PKD1

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBBR5pemIK

    Plasmid

    #126554

    Purpose

    Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection

    Depositor

    Lindsey Burbank, Drake Stenger

    Article

    Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAV0765

    Plasmid

    #133501

    Purpose

    Expression of mCherry targeted to the nucleus with one NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RCIscript-GoldyTALEN

    Plasmid

    #38142

    Purpose

    destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

    Depositor

    Daniel Carlson, Stephen Ekker

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Mrna transcription vector

    Tags

    AcV5 and FokI homodimer

    Expression

    Mutation

    Truncate at N and C terminous

    Promoter

    T3

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAV0532

    Plasmid

    #133502

    Purpose

    Expression of mTagBFP2 targeted to the nucleus with one NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAV0526

    Plasmid

    #133500

    Purpose

    Expression of sfGFP targeted to the nucleus with one NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. R4227X-F

    Plasmid

    #21371

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG (following D4226)

    Expression

    Mammalian

    Mutation

    Changed R4227 to stop codon

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

43. PB-UBC-mARG3

    Plasmid

    #173793

    Purpose

    Mammalian acoustic reporter gene 1 cassette 3 on PB-UBC plasmid

    Depositor

    Xiaojun Lian

    Article

    Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

    Insert

    GvpJ, F, G, L, K
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC promoter

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr

    Plasmid

    #19325

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Punc-122::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. zCD2

    Plasmid

    #122031

    Purpose

    Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Zebrafish HDAC6 Catalytic Domain 2
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pMM764

    Plasmid

    #68901

    Purpose

    IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Christopher Voigt, Timothy Lu

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    L51P

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAV0761

    Plasmid

    #133494

    Purpose

    Expression of nucleo-cytoplasmic mTagBFP2 in P-cells

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pMM733

    Plasmid

    #68898

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Christopher Voigt, Timothy Lu

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAV0661

    Plasmid

    #133476

    Purpose

    Expression in fission yeast from promoter pact1, integrated at ade6 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCS2-TagRFPT.zf1

    Plasmid

    #61390

    Purpose

    mRNA synthesis of TagRFPT codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    TagRFPT.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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