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We narrowed to 15,858 results for: cas9

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Showing: 881 - 900 of 15858 results

  1. pLdCH

    Plasmid
    #84291
    Purpose
    Express gRNA and Cas9 in Leishmania with Hygromycin resistance
    Depositor
    Greg Matlashewski
    Publication
    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA and Cas9
    Use
    CRISPR; Leishmania
    Promoter
    L. donovani ribosome RNA promoter
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    84291-plasmid-map-sequence-id-170879

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  2. pHelper_ShCAST_sgRNA

    Plasmid
    #127921
    Purpose
    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).
    Depositor
    Feng Zhang
    Publication
    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127921-plasmid-map-sequence-id-333562

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  4. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid
    #135013
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135013-plasmid-map-sequence-id-267601

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  6. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100982-plasmid-map-sequence-id-392326

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  8. pADH99

    Plasmid
    #90979
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Publication
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    Use
    CRISPR
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90979-plasmid-map-sequence-id-180505

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  9. pWS176

    Plasmid
    #90963
    Purpose
    Cas9 gap repair vector - ZeoR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    90963-plasmid-map-sequence-id-342430

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  10. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  14. pUS2-SH231

    Plasmid
    #115150
    Purpose
    Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4
    Depositor
    Raymond Monnat, Michael Phelps
    Publication
    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dual SH231 gRNAs
    Use
    CRISPR
    Tags
    3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    U6, CBh
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115150-plasmid-map-sequence-id-224718

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  15. lentiCRISPR - EGFP sgRNA 5

    Plasmid
    #51764
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    Use
    CRISPR and Lentiviral
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51764-plasmid-map-sequence-id-79386

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Showing: 881 - 900 of 15858 results