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pAAV-Tbg-CES2A-ΔC

Plasmid

#200558

Purpose

Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Promoter

TBG promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

Plasmid

#164131

Purpose

Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain

Plasmid

#79554

Purpose

Expression of catalytic domain of mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

aa990-1912

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5-3xFLAG-SRSF1 siRNA-resitant

Plasmid

#218974

Purpose

Vector for expressing siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGGAGUUUGUACGGAAA)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

SRSF1 cDNA

Expression

Mammalian

Mutation

siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGG…

Promoter

CMV

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-53BP1

Plasmid

#52507

Purpose

mammalian expression vector of 53BP1

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

V128A and siRNA resistant sequence: ACGCGCTGACGAC…

Promoter

CMV/TO

Available Since

March 26, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mRFP-FKBP12-5ptpase enzyme

Plasmid

#67515

Purpose

Recruitable human type IV 5-phosphatase enzyme

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Expression

Mammalian

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-MMLV-gag-hMLH1dn

Plasmid

#195513

Purpose

MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn (MLH1 Human)

Use

Virus like particle

Tags

FLAG, NES, and Protease cleavate site

Expression

Mammalian

Mutation

Human MLH1 del754-756

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
C1ORF43-GFP

Plasmid

#130879

Purpose

Expresses human C1ORF43 with C-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ikka-HA WT

Plasmid

#23296

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

IKK alpha (Chuk Mouse)

Tags

HA

Expression

Mammalian

Available Since

March 25, 2010

Availability

Academic Institutions and Nonprofits only
pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shSLC2A1-2

Plasmid

#193703

Purpose

Constitutive lentiviral expression of SLC2A1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSMPUW-miR-124-GFP-Puro

Plasmid

#117321

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Lentiviral

Tags

GFP-Puro

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Bhlhe40-3xFLAG-IRES-BFP

Plasmid

#117263

Purpose

Retroviral overexpression of Bhlhe40 with a 3xFLAG

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Bhlhe40 (Bhlhe40 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGL2-Mcl-1

Plasmid

#19132

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD59-T2A-GFP

Plasmid

#205460

Purpose

lentiviral plasmid for expression of human CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD59 (CD59 Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-mScarlet-I-CD4-bla

Plasmid

#179442

Purpose

Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHL-H1-ccdB-mEF1a-RiH

Plasmid

#60601

Purpose

Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

Red Fluorescent Protein
Hygromycin resistance gene

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-pDEST_BirA_Flag

Plasmid

#227960

Purpose

BioID experiment

Depositor

Alessandro Ori

Article

Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi

Insert

BirA

Expression

Mammalian

Mutation

R118G

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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