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pPD0039_CROP-seq-CAR-Puro

Plasmid

#242532

Purpose

CAR T screening

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CD19-BB CAR (CD19 Human)

Use

Lentiviral

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F1

Plasmid

#236431

Purpose

transient overexpression of E2F1 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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#2.4 pre-G-SynapShot-Myc

Plasmid

#214722

Purpose

Expresses green #2.4 pre-SynapShot and Myc tag

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#2.4 pre-G-SynapShot

Use

AAV

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMD2.G-H8A/K47Q

Plasmid

#207322

Purpose

VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSV-G (VSIVgp4 Vesicular Stomatitis Virus)

Expression

Mammalian

Mutation

H8A/K47Q

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pN006

Plasmid

#188470

Purpose

Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

AsCas12a

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-HypaCas9-mRuby2

Plasmid

#183872

Purpose

pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Type

Empty backbone

Use

CRISPR

Tags

HypaCas9-P2A-mRuby2-T2A-Puro

Expression

Mammalian

Promoter

Cbh

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_CACNB1_OE_1

Plasmid

#161660

Purpose

Overexpresses CACNB1

Depositor

Mike McManus

Insert

CACNB1 (CACNB1 Human)

Tags

V5

Expression

Mammalian

Available Since

March 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDRFLIP38-AtLEA4-5

Plasmid

#178189

Purpose

SED1 biosensor for expression in Saccharomyces cerevisiae

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Expression

Yeast

Promoter

pPMA1

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Sst22-GFP

Plasmid

#172312

Purpose

SST interneuron-restricted gene regulatory element GRE22, to drive GFP expression in SST+ interneurons

Depositor

Michael Greenberg

Article

Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

Insert

Sst22

Use

AAV

Promoter

pB-Globin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-TRE-ECC-Biosensor

Plasmid

#140833

Purpose

Encoding the TRE promoter based eccDNA biosensor

Depositor

Yonglun Luo

Article

Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

Insert

TRE-ECC Biosensor

Use

Piggybac (pb) transposon

Expression

Mammalian

Promoter

inverse

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPD304 VPR-ZF43

Plasmid

#138840

Purpose

Constitutive expression of VPR-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pStrep-1xEGFP-N1

Plasmid

#122488

Purpose

Expression of Strep-tagged 1xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 1xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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FnoCas12a-2C-NLS in pCSDest

Plasmid

#126639

Purpose

Expresses FnoCas12a-2C-NLS in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3RA-P2A-Puro

Plasmid

#110839

Purpose

Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Nanog-CreER targeting construct

Plasmid

#59720

Purpose

Cre-ER knockin targeting construct for mouse endogenous Nanog locus

Depositor

Jacob Hanna

Article

Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

Insert

creERT2

Use

Mouse Targeting

Promoter

mNanog

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFLuc190UGA

Plasmid

#41046

Depositor

James Inglese

Article

Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Firefly luciferase 190UGA

Expression

Mammalian

Mutation

in-frame UGA stop codon was created at codon 190

Promoter

CMV

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-SEC4

Plasmid

#18844

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

SEC4 (SEC4 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pRSET FLIPmal-25u

Plasmid

#14480

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

W230A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
U6_ISCro4_(WT)_TBL_bridge_RNA_polIII_term

Plasmid

#251496

Purpose

Expression of the Target Binding Loop (TBL) component of the native ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge RNA Target Binding Loop (TBL)

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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U6_ISCro4_(WT)_DBL_bridge_RNA_polIII_term

Plasmid

#251497

Purpose

Expression of the Donor Binding Loop (DBL) component of the native ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge RNA Donor Binding Loop (DBL)

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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