160,774 results
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Plasmid#45801DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1-IL2sp-6His-Asprosin plasmid
Plasmid#190683PurposeExpresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.DepositorInsertIL2-6xHis-Asprosin
UseTagsIL2 signal peptide, 6xHisExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-cell-mCer
Plasmid#185601PurposeLentiviral construct for expression of mCeruleanDepositorInsertcell-mCer
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET-His6-ERK2-MEK1_R4F_coexpression
Plasmid#39212DepositorUseTagsHis6 and RBSExpressionBacterialMutationMEK1(R4F) = S218E, S222D and deletion of AA32-51PromoterT7 and T7 via RBSAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_r5-HT1.0
Plasmid#208719PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neuronsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PAX3_FOXO1
Plasmid#205875PurposeExpress mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
PARP1 FL
Plasmid#169815PurposeExpresses codon optimised full length His tagged PARP1 in bacterial cellsDepositorInsertPoly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Synthetic, Human)
UseTagsMKHHHHHHMKQExpressionBacterialMutationValine 762 mutated to an alaninePromoterT7 promoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_UCA1
Plasmid#72628PurposeExpresses two gRNAs targeting the UCA1 promoterDepositorInsertgRNAs toward UCA1
UseTagsExpressionMammalianMutationPromoterU6 (gRNA1) and H1 (gRNA2)Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-eIF1a-GFP-RPL10a
Plasmid#159890PurposeExpresses GFP-RPL10a for ribosomal immunoprecipitationDepositorInsertRPL10a (RPL10A Human)
UseLentiviralTagsGFPExpressionMutationPromotereIF1aAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TetON-3G
Plasmid#96963PurposeMammalian expression of Clontech rtTA driven by human beta-actin promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo
Plasmid#128452Purposelentiviral destination vector with Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Avr2-GFP pZK538
Plasmid#118011Purposeexpresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .DepositorArticleInsertsdspAvr2
mCherry
UseTagsEGFP and HDELExpressionBacterial and PlantMutationPromoter35SAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-Flag-PALB2
Plasmid#71114Purposemammalian expession of FLAG-PALB2DepositorInsertPALB2 (PALB2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiFC-VN155(I152L)
Plasmid#27097DepositorInsertVN155(I152L)
UseTagsMycExpressionMammalianMutationI152LPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A
Plasmid#86674PurposeExpresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS E225A/D227A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
3XMEF2-luc
Plasmid#32967PurposeReporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoterDepositorInsertMEF2 binding sites (MEF2A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterMEF2Available sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAR17_StrepI_hFASh_H8_pET22b
Plasmid#122846PurposeExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInserthuman FASN/ human fatty acid synthase (FASN Human)
UseTagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3247
Plasmid#144723PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertLEF1 (LEF1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only