We narrowed to 15,384 results for: pET

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CMV-10xPP7 oligo-library based

Plasmid

#158199

Purpose

Cassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.

Depositor

Roee Amit

Article

Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

Insert

10 binding sites of PP7 from the oligo library

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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PAX6 CKO 2/7 Donor plasmid

Plasmid

#68467

Purpose

donor plasmid for targeting PAX6 locus

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

FRT flanked exon4 of PAX6 gene (PAX6 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg1 (CG509)

Plasmid

#139455

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pOPS0378

Plasmid

#133229

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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mADAM10 deltaMP in pQCXIP cFLAG

Plasmid

#19138

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM10 (Adam10 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 56-457

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pJT82_GalL_nCDA1Δ198-BE3

Plasmid

#145046

Purpose

Expresses nCDA1Δ198-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ198-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-198AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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Ad-Flag-HDAC3

Plasmid

#63675

Purpose

adenoviral expression of Flag tagged HDAC3

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

Insert

HDAC3 (Hdac3 Mouse)

Use

Adenoviral

Tags

FLAG

Expression

Mammalian

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pHS1477

Plasmid

#205970

Purpose

MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH all proteins, with His-tagged Cas11

Tags

14xHis on Cas11

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-NUbo-GFP-His6

Plasmid

#212790

Purpose

Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-GFP-His6

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 left TALE–G1333 DddAtox C

Plasmid

#158092

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-CTRLg1

Plasmid

#139450

Purpose

Lentiviral vector with non-targeting gRNA and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pVRa22_4450

Plasmid

#49676

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF22_4450

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-ITFG2

Plasmid

#87049

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pVRa03_1198

Plasmid

#49655

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF03_1198

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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ND4 right TALE–G1333 DddAtox N

Plasmid

#158096

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3

Plasmid

#120253

Purpose

Expression of ATP3 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgAtm/Cre

Plasmid

#89643

Purpose

Expresses an Atm-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Atm (Atm Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-MYC-ITFG2

Plasmid

#87046

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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PAX6 sgRNA7

Plasmid

#68466

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-C–SaKKH-Cas9(D10A)

Plasmid

#157838

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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