We narrowed to 11,712 results for: NSI

1. pLKO_NWS.AU1.V5_NGFR

   Plasmid

   #158237

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   NWS.AU1.V5

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-flag-RAP1B-N17-puro

   Plasmid

   #156169

   Purpose

   Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-PGK-RAP2A-WT-puro

   Plasmid

   #156170

   Purpose

   Lentiviral vector for expression of RAP2A-WT, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-RAP2A-V12-puro

   Plasmid

   #156171

   Purpose

   Lentiviral vector for expression of RAP2A-V12, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Valine

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PGK-RAP2A-N17-puro

   Plasmid

   #156172

   Purpose

   Lentiviral vector for expression of RAP2A-N17, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGMC00001

   Plasmid

   #166718

   Purpose

   sgRNA against human BAP1

   Depositor

   Raj Chari

   Article

   Rathkey et al J Thorac Oncol. 2020 May;15(5):843-859.

   Insert

   BAP1 (BAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGMC00002

   Plasmid

   #166719

   Purpose

   sgRNA against human BAP1

   Depositor

   Raj Chari

   Article

   Rathkey et al J Thorac Oncol. 2020 May;15(5):843-859.

   Insert

   BAP1 (BAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-FOXI3

   Plasmid

   #101518

   Purpose

   Donor Vector containing FOXI3 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   FOXI3 (FOXI3 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-ASCL3

   Plasmid

   #101515

   Purpose

   Donor Vector containing ASCL3 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ASCL3 (ASCL3 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221-HOXB3

    Plasmid

    #101478

    Purpose

    Donor Vector containing HOXB3 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-FOXE1

    Plasmid

    #101439

    Purpose

    Donor Vector containing FOXE1 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    FOXE1 (FOXE1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221-FOXE3

    Plasmid

    #101429

    Purpose

    Donor Vector containing FOXE3 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    FOXE3 (FOXE3 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221-FOXH1

    Plasmid

    #101431

    Purpose

    Donor Vector containing FOXH1 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    FOXH1 (FOXH1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221-BSX

    Plasmid

    #101410

    Purpose

    Donor Vector containing BSX transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    BSX (BSX Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221-HOXB9

    Plasmid

    #101398

    Purpose

    Donor Vector containing HOXB9 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    HOXB9 (HOXB9 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221-HOXC5

    Plasmid

    #101399

    Purpose

    Donor Vector containing HOXC5 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    HOXC5 (HOXC5 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221-HOXD8

    Plasmid

    #101402

    Purpose

    Donor Vector containing HOXD8 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    HOXD8 (HOXD8 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-ETV3

    Plasmid

    #88804

    Purpose

    Donor Vector containing ETV3 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ETV3 (ETV3 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR1A_mmFbxw7_R505C (closed)

    Plasmid

    #160103

    Purpose

    Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Fbxw7 alpha R505C (Fbxw7 Mouse)
    

    Use

    Gateway entry vector

    Expression

    Mammalian

    Mutation

    R505C

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAav-TP53-PQS1-3xFLAG

    Plasmid

    #84884

    Purpose

    rAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Inserts

    TP53 genomic region HR1 (TP53 Human)
    TP53 genomic region HR2
    

    Use

    AAV

    Tags

    PQS1 3xFLAG

    Promoter

    no and no

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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