We narrowed to 9,184 results for: Pol

1. pLX304-EZH1-D745N

   Plasmid

   #203585

   Purpose

   Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

   Depositor

   William Kaelin, Abhishek Chakraborty

   Article

   Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

   Insert

   EZH1 (EZH1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 745 to Asparagine for parti…

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTW5126

   Plasmid

   #129732

   Purpose

   polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

   Insert

   eatA-DUF::His8
   

   Tags

   9His tag

   Expression

   Bacterial

   Mutation

   replaces the domain of unknown function correspon…

   Promoter

   araBAD

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169162

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
   

   Tags

   6His-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

   Plasmid

   #169163

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
   

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-LOC442075

   Plasmid

   #23372

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   LOC442075 (LOC442075 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pCMV4 p65

   Plasmid

   #21966

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NFkB p65 subunit (RELA Human)
   

   Expression

   Mammalian

   Mutation

   This construct was the product of 3 subcloning pr…

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-PDGFRA

   Plasmid

   #23892

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PDGFRA (PDGFRA Human)
   

   Use

   Gateway donor vector

   Mutation

   M260I

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-TetOminiCMV-OSK

   Plasmid

   #136552

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-CSNK2A1

   Plasmid

   #23678

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   CSNK2A1 (CSNK2A1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-CMV-Puro-PNPT1 S484A

    Plasmid

    #223316

    Purpose

    Lentiviral vector expressing human PNPT1 S484A variant

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed serine 484 to alanine

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-PDGFRB

    Plasmid

    #23893

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-CSNK2B

    Plasmid

    #23359

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CSNK2B (CSNK2B Human)
    

    Use

    Gateway donor vector

    Mutation

    C138T

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pAB2076 pAAV REPAIR.t1

    Plasmid

    #176323

    Purpose

    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    

    Use

    AAV and CRISPR

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    E488Q, only the deaminase domain (aa 276-702 are …

    Promoter

    EFS (short EF1alpha) and hU6

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAd-M3cherry

    Plasmid

    #61041

    Purpose

    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.

    Depositor

    Qiao Zhou

    Article

    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.

    Inserts

    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    

    Use

    Adenoviral

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-PIK3CA

    Plasmid

    #23466

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Gateway donor vector

    Mutation

    A1035V

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pGL4.23-CD69 CaRE3 (minus strand)

    Plasmid

    #91845

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0622)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE3 (minus strand) (CD69 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Missing 1 base from 13 base polyT tract beginning…

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-TetOminiCMV-SKM

    Plasmid

    #136551

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

    Plasmid

    #231549

    Purpose

    Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Tags

    msfGFP (intramolecular)

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMVtrunc msfGFPΔC10-beta-actin

    Plasmid

    #231548

    Purpose

    Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223-RPS6KB2

    Plasmid

    #23533

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    RPS6KB2 (RPS6KB2 Human)
    

    Use

    Gateway donor vector

    Mutation

    R482Stop

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only