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P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

Plasmid

#70211

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deCFP

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS285

Plasmid

#73734

Purpose

SapTrap egl-13 NLS connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

egl-13 NLS

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMV-HIS3

Plasmid

#65333

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

HIS3

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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SepOTSγ

Plasmid

#68284

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 1xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-C1

Plasmid

#54854

Purpose

Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

tdminiSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
tdminiSOG-N1

Plasmid

#54855

Purpose

Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

tdminiSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCN34e-ktrA-yfp

Plasmid

#227640

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCN34e-kimA-yfp

Plasmid

#227638

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_AchLightR

Plasmid

#187463

Purpose

Expresses red acetylcholine indicator AchLightR in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AchLightR

Use

AAV

Tags

Igκ leader

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-mGreenLantern

Plasmid

#197922

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern

Expression

Mammalian

Mutation

none

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEY17

Plasmid

#191023

Purpose

flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-18 CELE_Y48D7A.2 (flp-18 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_INSR

Plasmid

#187788

Purpose

MAC-tagged gene expression of human INSR

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

INSR (INSR Human)

Expression

Mammalian

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-iGECInano

Plasmid

#186189

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Bacterial

Promoter

AraC

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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3TERM_35S

Plasmid

#136114

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFTSG

Plasmid

#162389

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

His

Expression

Insect

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TwinStrep-GFP11

Plasmid

#153514

Purpose

TwinStrep-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TwinStrep-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_His2-GFP11

Plasmid

#153511

Purpose

His2-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

His2-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-rev_U6_MCS

Plasmid

#109769

Purpose

Multisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

U6-gRNA cassette

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pKM463

Plasmid

#134214

Purpose

Baculovirus expression of CENP-U and CENP-Q

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENP-U + Q

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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