162,408 results

1. STAT3(Y705F)-TAL-Luc

   Plasmid

   #46933

   Purpose

   Luciferase Reporter for mutant STAT3

   Depositor

   Afshin Dowlati

   Article

   Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

   Insert

   STAT3(Y705F) (STAT3 Human)
   

   Use

   Luciferase

   Tags

   FLAG

   Expression

   Mutation

   Tyrosine 705 to Phenylalanine

   Promoter

   TA Luciferase

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-FLEX-SaCas9-U6-sgSlc17a6

   Plasmid

   #124847

   Purpose

   Mutagenesis of Slc17a6 with SauCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 gRNA (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Plasmid L5 attB::Pleft* mWasabi

   Plasmid

   #169409

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mWasabi
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAVS1-CAG-hrGFP

   Plasmid

   #52344

   Purpose

   AAVS1 donor vector for constitutive gene expression

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   hrGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGK1p-Csy4-pA (Construct 2)

   Plasmid

   #55196

   Purpose

   Expresses Csy4 in Mammalian cells for site-specific RNA processing

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   Csy4/Cas6
   

   Use

   Synthetic Biology

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS-NICD

   Plasmid

   #26891

   Depositor

   Nicholas Gaiano

   Article

   Dang et al Dev Neurosci. 2006;28(1-2):58-69.

   Insert

   NICD (Notch1 Mouse)
   

   Use

   Tags

   FLAG and b-globin polyA

   Expression

   Mammalian

   Mutation

   E2299K

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

7. TFORF0214

   Plasmid

   #142486

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXP1 (FOXP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC18T-mini-Tn7T-hph-Ptet1

   Plasmid

   #218367

   Purpose

   Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti PGK V5-LUC Puro (w543-1)

   Plasmid

   #19360

   Purpose

   3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-CalfluxVTN

    Plasmid

    #83926

    Purpose

    Bioluminescent Reporter of Intracellular Calcium

    Depositor

    Carl Johnson

    Article

    Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

    Insert

    CalfluxVTN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2 HRS-RFP

    Plasmid

    #29685

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223-PRKAG3

    Plasmid

    #23549

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pGP-CMV-jGCaMP7s

    Plasmid

    #104463

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7s
    

    Use

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. HH-gRNA-HDV-Shuttle-Vector

    Plasmid

    #169098

    Purpose

    Backbone plasmid to generate HH-gRNA-HDV segment

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Development. 2021 Mar 9. pii: dev.193565

    Insert

    HH-gRNAbackbone-HDV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NA

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pORANGE GFP-Grin1 KI #3

    Plasmid

    #139659

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF2242

    Plasmid

    #141986

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBTK1008 (pSL7)

    Plasmid

    #191003

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG_PCP-T5Exo

    Plasmid

    #214736

    Purpose

    encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_PCP-T5Exo_bGH
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CLYBL-TO-PAX7

    Plasmid

    #204346

    Purpose

    Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human PAX7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV_hEF1a_rtTA3

    Plasmid

    #61472

    Purpose

    3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Insert

    rtTA3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hEF1a

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge