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pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

Plasmid

#205631

Purpose

Expression of TnpBmax in mammalian cells

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

ISDra2-TnpB optimized for mammalian expression

Tags

NLS-3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

Plasmid

#233080

Purpose

Expression vector for sgRNA-S1b and eCas12f1i

Depositor

Kyoungmi Kim

Article

Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

Insert

U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP

Use

CRISPR

Tags

2xFLAG

Expression

Mammalian

Promoter

hU6, CBh

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12982 - pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2982)

Plasmid

#191709

Purpose

AI plasmid ALIAS: AiP12982. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-HaloDA1.0

Plasmid

#234408

Purpose

Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 in neurons

Depositor

Yulong Li

Article

Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

Insert

GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0

Use

AAV

Promoter

hSyn

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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Talin1-mCherry

Plasmid

#212004

Purpose

Expression of mouse full-length (1-2541) talin1-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

Insert

Mouse talin1 (Tln1 Mouse)

Tags

mCherry fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-V5

Plasmid

#207686

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCG004

Plasmid

#87377

Purpose

ORF part assembly entry vector derived from pHT01.

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

GFP dropout part

Expression

Bacterial

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLENTI_hACE2_PURO

Plasmid

#155295

Purpose

Lentiviral vector to generate hACE2 stable expressing cell line

Depositor

Raffaele De Francesco

Insert

hACE2 (ACE2 Human)

Use

Lentiviral

Tags

C9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

Plasmid

#183775

Purpose

AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

NLS-GFP

Use

AAV

Mutation

NA

Promoter

CAG

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF3084

Plasmid

#144560

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZFP36L1 (ZFP36L1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Plasmid

#132543

Purpose

Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

Plasmid

#160141

Purpose

Cre- dependent expression of Sun1GFP for nuclei isolation

Depositor

Andreas Pfenning

Article

Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

Insert

Sun1-GFP (Sun1 Mouse)

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

N-truncated: removed amino acids 1-207, retained …

Promoter

Ef1a

Available Since

March 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWBs02

Plasmid

#222478

Purpose

Gateway vector for 35s promoter-driven expression in Marchantia polymorpha carrying a sulfadiazine selection marker.

Depositor

Philip Carella

Article

Robinson et al J Exp Bot. 2024 Jun 2:erae256. doi: 10.1

Type

Empty backbone

Expression

Plant

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300

Plasmid

#89094

Purpose

mammalian expression of full-length human p300 in pSG5

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-3xHA

Plasmid

#207685

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC39A7_STOP

Plasmid

#161331

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A7 (SLC39A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLENTI-CMV-GFP-TREX1 BLAST

Plasmid

#164228

Purpose

lentiviral plasmid for GFP-hTREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12D

Plasmid

#81651

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

G12D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pTH100

Plasmid

#84458

Purpose

pJB38-NWMN29-30 + SarA_P1-sGFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-sGFP from pCM29

Tags

6xHis (on sfGFP)

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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