159,617 results

1. TFORF0969

   Plasmid

   #141770

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HAND2 (HAND2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. WFS1-Flag-pcDNA3

   Plasmid

   #13011

   Depositor

   Fumihiko Urano

   Article

   Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

   Insert

   Wolfram Syndrome 1 (WFS1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJUMP23-1A(sfGFP)

   Plasmid

   #126970

   Purpose

   Level 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa-LSS-GFP-LDLR wt

   Plasmid

   #98184

   Purpose

   Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

   Insert

   LDLR (LDLR Human)
   

   Use

   Tags

   GFP and LDLR signal sequence, N-term of GFP so GF…

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Chicken Beta Actin

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLSSmKate2-N1

   Plasmid

   #31867

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   LSS-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V

   Promoter

   CMV

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. JME4599

   Plasmid

   #129660

   Purpose

   NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT29

   Plasmid

   #138521

   Purpose

   phage cloning

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   phage genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. VCP(DK0)-EGFP

   Plasmid

   #23974

   Depositor

   Nico Dantuma

   Article

   Tresse et al Autophagy. 2010 Feb 22. 6(2):.

   Insert

   Valosin containing protein (VCP Human)
   

   Use

   Tags

   6xHis, EGFP, and HA

   Expression

   Mammalian

   Mutation

   E305QE578Q

   Promoter

   Available since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-puro-EWS-FLI1

    Plasmid

    #102813

    Purpose

    Overexpress EWS-FLI1. 3rd generation lentiviral vector.

    Depositor

    Jialiang Wang

    Article

    Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

    Insert

    EWS-FLI1 (EWSR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CL20_mEGFP_KMT2A_MLLT1

    Plasmid

    #205842

    Purpose

    Express mEGFP-tagged fusion protein, KMT2A_MLLT1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    KMT2A_MLLT1 (KMT2A, MLLT1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mRFP1-N1

    Plasmid

    #54635

    Purpose

    Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

    Depositor

    Roger Tsien, Robert Campbell, Michael Davidson

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

    Type

    Empty backbone

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-flpE-puro

    Plasmid

    #20733

    Purpose

    Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    FLPe recombinase
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbA2k-RFP

    Plasmid

    #35327

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-ERBB4

    Plasmid

    #29527

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

16. TRBV5-1

    Plasmid

    #123414

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV5-1 (TRBV5-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-EF1a-dCas9-VP64-Puro

    Plasmid

    #99371

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-VP64-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-S5E2-Gq-P2A-dTomato

    Plasmid

    #135635

    Purpose

    AAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    Gq-P2A-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)/Luc2=tdT

    Plasmid

    #32904

    Purpose

    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

    Depositor

    Christopher Contag

    Article

    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

    Inserts

    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    

    Use

    Tags

    tdTomato red fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE and n/a

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. NLS-Halo

    Plasmid

    #200197

    Purpose

    Expression for SV40 NLS

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    3X SV40 NLS-Halo
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge