We narrowed to 17,813 results for: puro
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Plasmid#200946PurposeEncodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporationDepositorInsertsUseLentiviralTags6x HISMutationamber stop codon 101 bp from ATGPromoterEf1a and hPGKAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only
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VEGF164TAG
Plasmid#200947PurposeEncodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporationDepositorInsertsUseLentiviralTags6x HISMutationamber stop codon 101 bp from ATGPromoterEf1a and hPGKAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:NEAT1-total_SigP:NLuc
Plasmid#197267PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertNEAT1-total homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…ExpressionMammalianAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-CuR-hSOD1
Plasmid#232477PurposeThe inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' endDepositorInsertHomo sapiens superoxide dismutase 1 (SOD1 Human)
UseTransposon systemTagsDYKDDDDK (FLAG-tag)ExpressionMammalianPromoterCMV-CuOAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-TR-hSOD1
Plasmid#232478PurposeTetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' endDepositorInsertHomo sapiens superoxide dismutase 1 (SOD1 Human)
UseTransposon systemTagsDYKDDDDK (FLAG-tag)ExpressionMammalianPromoterCMV-TetOAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-DIO-Magneto2.0-sNRPpA
Plasmid#74307PurposeCre-dependent expression of Magneto2.0 in mammalian cellsDepositorUseAAV and Cre/LoxTagsFlagExpressionMammalianMutationTRPV4: delta 760-871PromoterCMV (after Cre recombination)Available SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pLenti_CMVTRE3G_LYSET-Isoform2_Flag
Plasmid#191999PurposeLentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoterDepositorAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA
Plasmid#74302PurposeExpression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafishDepositorUseZebrafishTagsFLAG, cmcl2::EGFP, and p2A-mCherryMutationTRPV4: delta 760-871PromoterNeurog1Available SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA
Plasmid#74306PurposeBicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cellsDepositorUseAAV and Cre/LoxTagsFLAG and p2AExpressionMammalianPromoterCMV (after cre recombination)Available SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGLUE-SHLD2
Plasmid#114118PurposeExpresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cellsDepositorInsertSHLD2 (SHLD2 Human)
TagsHA tag, Streptavidin-binding tag (Streptag), and …ExpressionMammalianPromoterCMVAvailable SinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
BAP1-C91A
Plasmid#108440PurposeStable expression of BAP1-C91A in mammalian cells.DepositorAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag
Plasmid#192006PurposeLentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoterDepositorInsertLYSET-Isoform2-I142V-Flag (LYSET Human)
UseLentiviralTagsFlagExpressionMammalianPromoterCMVTRE3GAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag
Plasmid#192005PurposeLentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoterDepositorInsertLYSET-Isoform2-R45W (LYSET Human)
UseLentiviralTagsFlagExpressionMammalianPromoterCMVTRE3GAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196084Purpose(Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseCRISPRExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-ASCL1-DLX2
Plasmid#182307PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expressionTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and pTRE3G-bidirectionalAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196078Purpose(Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
ExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
BAP1-wt (siRNA resistant)
Plasmid#108439PurposeStable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.DepositorInsertBAP1 (BAP1 Human)
ExpressionMammalianMutationc.1641C>T, c.1644T>A, c.1647T>C, c.1650C…PromoterCMVAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
BAP1-C91A (siRNA resistant)
Plasmid#108438PurposeStable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.DepositorInsertBAP1 (BAP1 Human)
ExpressionMammalianMutationC91A: c.271T>G, c.272G>C; others: c.1641C&g…PromoterCMVAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only