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Showing: 9121 - 9140 of 68612 results

  4. pLL7 lenti-dcas9-3xflag-blast

    Plasmid
    #112133
    Purpose
    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)
    Depositor
    Fei-Long Meng
    Publication
    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.
    Insert
    dCas9(D10A, N863A)-T2A-Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A mutants in Cas9
    Promoter
    EF1a
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112133-plasmid-map-sequence-id-222675

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  5. AICSDP-45: TTN-mEGFP

    Plasmid
    #114412
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114412-plasmid-map-sequence-id-220885

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  7. pX458-ECFP

    Plasmid
    #112220
    Purpose
    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP
    Depositor
    Yinming Liang
    Publication
    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ECFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    112220-plasmid-map-sequence-id-215204

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  15. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid
    #107939
    Purpose
    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.
    Depositor
    Heng-Chi Lee
    Publication
    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d
    Insert
    mCherry(dpiRNA)::ANI-1(AHPH)
    Expression
    Worm
    Promoter
    pie-1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107939-plasmid-map-sequence-id-207466

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  19. pTO_CHMP3_LAP

    Plasmid
    #101849
    Purpose
    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Publication
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101849-plasmid-map-sequence-id-200249

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Showing: 9121 - 9140 of 68612 results