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Showing: 9121 - 9140 of 65888 results

  1. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    131300-plasmid-map-sequence-id-254651

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  7. mAC-POLR2A donor (Hygro)

    Plasmid
    #124496
    Purpose
    A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover
    Depositor
    Masato Kanemaki
    Publication
    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
    Use
    CRISPR
    Tags
    mAID-mClover
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    124496-plasmid-map-sequence-id-243723

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  13. GG-EBNA-EEA-5guides-PGK-Puro

    Plasmid
    #102898
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EEA-5guides-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    102898-plasmid-map-sequence-id-198364

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  14. pDNA2.0 6H-TEV-KRAS G13D

    Plasmid
    #111851
    Purpose
    Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Publication
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G13D (KRAS Human)
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 13 to Aspartic Acid
    Promoter
    T5
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111851-plasmid-map-sequence-id-213465

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  19. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid
    #102851
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3X Flag and NLS
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102851-plasmid-map-sequence-id-198995

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Showing: 9121 - 9140 of 65888 results