We narrowed to 24,060 results for: crispr

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pYPQ146-ZmUbi-tRNA

Plasmid

#158404

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-H2BC11

Plasmid

#207755

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)

Plasmid

#71814

Purpose

Expresses high specificity SpCas9. Px330-like plasmid.

Depositor

Feng Zhang

Article

Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

Has Service

Cloning Grade DNA

Insert

enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pM378

Plasmid

#173174

Purpose

Expresses C9-1 gRNA-12xMBS from hU6 promoter

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

C9-1 targeting gRNA-12xMBS

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCH49 (crRNA expression vector)

Plasmid

#217338

Purpose

CROP-seq crRNA expression vector with GFP and neo marker

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

AsDR-filler-AsDR8, GFP-neo

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDuBir-Lbu-dCas13a-avitag

Plasmid

#100817

Purpose

Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

Depositor

Mitchell O'Connell

Article

Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

Insert

Lbu_Cas13a_R472A_H477A_R1048A_H1053A

Tags

AviTag and His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Promoter

T7

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ134B2.0

Plasmid

#167158

Purpose

Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-IBM1 (D30)

Plasmid

#239492

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Promoter

DHFR-TS 5' UTR

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCAT005

Plasmid

#171632

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-B2M
mNeon

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1-a and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TetON-dual-SunTag-Hygro

Plasmid

#121119

Purpose

Endogenous gene activation with the SunTag CRISPR activation system

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

dCas9-GCN4x10_scFv-VP64

Use

CRISPR

Expression

Mammalian

Promoter

Tet-ON

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ132B2.0

Plasmid

#99888

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ131B2.0

Plasmid

#99885

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-PGK- dCas9-SunTag-P2A-HygR

Plasmid

#193137

Purpose

Expresses dCas9 fused to the SunTag scaffold

Depositor

Brad Rosenberg

Article

Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

Insert

dCas9-SunTag-T2A-HygR

Use

Lentiviral

Promoter

PGK

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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ROS1-dCas9 (D10)

Plasmid

#239487

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-ROS1-XTEN80-NLS-dCas9-2xNLS-P2A-BFP

Use

CRISPR

Expression

Plant

Available Since

Feb. 17, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-BFP

Plasmid

#78547

Purpose

Cas9 protein with BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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PB-CMV-NLS-dCas9-NLS

Plasmid

#99908

Purpose

For the expression of NLS-dCas9-NLS

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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