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pcDNA6-MRPL50-VC155

Plasmid

#182373

Purpose

mVenus-based mito-riboBiFC vector (Negative control)

Depositor

Chunghun Lim

Publication

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

Mrpl50 (Mrpl50 Mouse)

Tags

VC155

Expression

Mammalian

Promoter

CMV promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG

Plasmid

#178861

Purpose

Mammalian expression of PB1-AzamiGreen

Depositor

Shinya Tsukiji

Publication

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PB1-AzamiGreen

Expression

Mammalian

Promoter

CMV

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-AMBRA1-3xFLAG

Plasmid

#174159

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1A

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG CPTP

Plasmid

#170742

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Publication

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CPTP (CPTP Human)

Tags

FLAG

Expression

Mammalian

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)

Plasmid

#160607

Purpose

TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.

Depositor

Diego Orzaez

Insert

CUP2Gal4AD

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hRBM34-HA

Plasmid

#165464

Purpose

Human RBM34 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Publication

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

RNA binding motif protein 34 (RBM34 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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IX_pAAV-ProB3-CatCh-GFP-WPRE

Plasmid

#125923

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GFP

Use

AAV

Promoter

ProB3

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS2

Plasmid

#109289

Purpose

Expresses high levels of protein in mycobacteria

Depositor

Nicolai van Oers

Publication

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Type

Empty backbone

Expression

Bacterial

Mutation

pSUM-kan-MCS1-gfp was modified to reorient the Ba…

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pWL1 (mANGPTL8-V5-His)

Plasmid

#109113

Purpose

Expresses V5 and His-tagged mouse ANGPTL8 in mammalian cells

Depositor

Brandon Davies

Publication

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL8 (Angptl8 Mouse)

Tags

6x His and V5

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLX302-PTP-SL-V5 puro

Plasmid

#87774

Purpose

Expresses V5 tagged human PTP-SL

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

PTP-SL (PTPRR Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12C

Plasmid

#87080

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Publication

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM12C (Trim12c Mouse)

Expression

Mammalian

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

no stop codon

Promoter

none

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pHcPro (GB0067)

Plasmid

#68196

Purpose

Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

TEV HcPro coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pSG 417

Plasmid

#63864

Purpose

Detect translation initiation from AUU start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Publication

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUU start codon

Promoter

PtacI

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pSG 416

Plasmid

#63863

Purpose

Detect translation initiation from AUA start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Publication

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUA start codon

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 T538N

Plasmid

#60537

Purpose

Donor vector for human Pak5 T538N mutant

Depositor

John Brognard

Publication

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway Cloning

Mutation

T538N

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S573N

Plasmid

#60538

Purpose

Donor vector for human Pak5 S573N mutant

Depositor

John Brognard

Publication

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway Cloning

Mutation

S573N

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pICSL30004

Plasmid

#50302

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CDS no stop codon, YFP variant of GFP (Aequorea victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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VIPR2

Plasmid

#39044

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

VIPR2 (VIPR2 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
p4xTRE1-Luc

Plasmid

#16535

Depositor

Bert Vogelstein

Publication

He et al Cell. 1999 Oct 29. 99(3):335-45.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

4xTRE1

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

May 12, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

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