159,557 results

1. PLKO-mcherry-luc-puro-TRPM1 cDNA

   Plasmid

   #29782

   Depositor

   Carl Novina

   Article

   Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

   Insert

   TRPM1 (TRPM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCW01

   Plasmid

   #72300

   Purpose

   Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Type

   Empty backbone

   Use

   Derivative of puc19

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-HP1alpha

   Plasmid

   #179703

   Purpose

   visualization of centromere heterochromatin

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   heterochromatin protein alpha (CBX5 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET his-eGFP

   Plasmid

   #113551

   Purpose

   For bacterial expression and purification of eGFP

   Depositor

   Jeanne Stachowiak

   Article

   Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

   Insert

   his-eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A206K

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPIPL12-FnCas12a

   Plasmid

   #199563

   Purpose

   Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

   Depositor

   Philippe Lambin

   Article

   Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

   Insert

   Fncas12a
   

   Use

   CRISPR and Synthetic Biology; Tetracycline induci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   COtetR-PIPL12

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPfetFn-Target_v2

   Plasmid

   #199564

   Purpose

   gRNA expression for FnCas12a in E. coli and clostridia

   Depositor

   Philippe Lambin

   Article

   Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

   Insert

   SacB
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pthl_fU

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2 RFP670

   Plasmid

   #187646

   Purpose

   lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site

   Depositor

   Martin Carroll

   Insert

   RFP 670
   

   Use

   Lentiviral

   Tags

   RFP670 and sgRNA cloning site

   Expression

   Mutation

   Promoter

   EFS (P2A)

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-HP1alpha

   Plasmid

   #179701

   Purpose

   visualization of centromere heterochromatin

   Depositor

   Kenji Sugimoto

   Article

   Yamada et al J Biochem. 1999 Apr . 125(4):832-7.

   Insert

   heterochromatin protein alpha (CBX5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC1A2

    Plasmid

    #131901

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A2 (SLC1A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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