165,187 results

1. pCS2-Jmjd3-F

   Plasmid

   #17440

   Purpose

   Expression of Jmjd3 in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   JMJD3 (Kdm6b Mouse)
   

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. YopH (residues 164-468)

   Plasmid

   #79749

   Purpose

   This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   YopH (residues 164-468)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCB-hDot1Lmut

   Plasmid

   #74174

   Purpose

   For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

   Depositor

   Yi Zhang

   Article

   Okada et al Cell. 2005 Apr 22;121(2):167-78.

   Insert

   hDot1L (DOT1L Human)
   

   Use

   Retroviral

   Tags

   HA

   Mutation

   hDot1L 163 GSG to RCR mutation*

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

4. miniTurbo-V5_pRS415

   Plasmid

   #107168

   Purpose

   expresses V5-tagged miniTurbo in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Yeast

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p21-Reporter

   Plasmid

   #52432

   Purpose

   DNA damage inducible Red Fluorescent Protein for mammalian expression

   Depositor

   Joel Voldman

   Article

   Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

   Insert

   p21-p53 Tandem Response Element Promoter
   

   Tags

   TurboRFP

   Expression

   Mammalian

   Promoter

   13X repeated p21-p53 response element

   Available Since

   Oct. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

   Plasmid

   #169283

   Purpose

   Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

   Depositor

   Dirk Heckl

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5 FRT TO GFP11-LRRK2_G2019S

   Plasmid

   #231175

   Purpose

   Expresses GFP-11-LRRK2 G2019S in mammalian cells

   Depositor

   Samara Reck-Peterson

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   GFP11-LRRK2 (G2019S) (LRRK2 Human)
   

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   G2019S

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPYtag-Histag-Avidin

   Plasmid

   #214836

   Purpose

   This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

   Insert

   SPYtag-Histag-Avidin
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MEK1-GFP

   Plasmid

   #14746

   Depositor

   Rony Seger

   Article

   Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

   Insert

   MEK1 (MAP2K1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+mScarlet3-C Cloning Vector

    Plasmid

    #220616

    Purpose

    To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    mScarlet3 fusion

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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