We narrowed to 18,937 results for: Tec

1. OSR1 gRNA (BRDN0001145611)

   Plasmid

   #76148

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human OSR1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   OSR1 (OSR1, OXSR1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. OSR1 gRNA (BRDN0001145425)

   Plasmid

   #76149

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human OSR1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   OSR1 (OSR1, OXSR1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PI4K2A gRNA (BRDN0001146485)

   Plasmid

   #76820

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PI4K2A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PI4K2A (PI4K2A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PI4K2A gRNA (BRDN0001146736)

   Plasmid

   #76821

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PI4K2A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PI4K2A (PI4K2A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SIK2 gRNA (BRDN0001148975)

   Plasmid

   #77139

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SIK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SIK2 (SIK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PRKAR1A gRNA (BRDN0001146329)

   Plasmid

   #77720

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKAR1A (PRKAR1A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NADK2 gRNA (BRDN0001147240)

   Plasmid

   #78092

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NADK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NADK2 (NADK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PET-SpCas9-HF1-plus-NLS-6xHis

   Plasmid

   #207381

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, Q695A, Q926A, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PET-B-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207385

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MLM3762

    Plasmid

    #49947

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x FLAG Tet1CDmut RH-4 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

    Plasmid

    #163571

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MTOR gRNA (BRDN0001145868)

    Plasmid

    #76784

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MTOR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PDGFRB gRNA (BRDN0001147806)

    Plasmid

    #77059

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PDGFRB

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX307-MERS-CoV-3CL-C148A

    Plasmid

    #160283

    Purpose

    Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C148A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PRKD2 gRNA (BRDN0001147099)

    Plasmid

    #77614

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKD2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKD2 (PRKD2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RPS6KA5 gRNA (BRDN0001146427)

    Plasmid

    #77373

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA5

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA5 (RPS6KA5 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PRKD1 gRNA (BRDN0001144834)

    Plasmid

    #77230

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKD1 (PRKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCHKO_TK1_HPRT1_Lb_1

    Plasmid

    #155059

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123337

    Purpose

    Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Lyn-Venus-cpVenus-FLARE-AKAR
    

    Tags

    N-terminal targeting sequence from Lyn kinase, Ve…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NADK2 gRNA (BRDN0001146214)

    Plasmid

    #78091

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NADK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NADK2 (NADK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge