We narrowed to 16,820 results for: URE

1. pFOSGE_COF2F04

   Plasmid

   #70901

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   mod(mdg4) (mod(mdg4) Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

2. PB-tetO-hNIL

   Plasmid

   #197089

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Martin S. Taylor, Oliver Weichenrieder

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / Lac

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

   Plasmid

   #154088

   Purpose

   Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet on

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pINDUCER-21-RUNX1-P2A-ERG-IMPROVED

   Plasmid

   #154089

   Purpose

   Improved version to express RUNX1, P2A, ERG in mammalian cells.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Runt Related Transcription Factor 1, ETS-Related Gene (RUNX1, ERG Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet on

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

   Plasmid

   #217014

   Purpose

   Expression of CDK2-7 chimera in E.coli

   Depositor

   Manjeet Mukherjee

   Article

   Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

   Insert

   CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)
   

   Use

   Tags

   C3 protease cleavable GST tag

   Expression

   Bacterial

   Mutation

   CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

   Plasmid

   #213030

   Purpose

   Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

   Plasmid

   #213031

   Purpose

   Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

   Depositor

   Martin S. Taylor, Donna Romero

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
   

   Use

   Luciferase

   Tags

   Firefly luciferase antisense intron (3' UTR)

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE bidirectional tet-on

   Available since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

   Plasmid

   #213026

   Purpose

   Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Split 'n coexpress CUL3-RBX1

    Plasmid

    #52293

    Purpose

    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Inserts

    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    

    Use

    Tags

    GST, IRES, and TEV

    Expression

    Bacterial

    Mutation

    contains aa 1-309, contains aa 310-768, and conta…

    Promoter

    IRES and tac

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

    Plasmid

    #213025

    Purpose

    Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (1-1275)
    

    Use

    Tags

    3C-3xFlag (ORF2p)

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHis-TEV-Nsp15

    Plasmid

    #187656

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

    Insert

    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-BSD-tet-mEGFP-MIB1

    Plasmid

    #140241

    Purpose

    Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Tet-inducible mEGFP-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. JGI_018129

    Plasmid

    #158934

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_108826550_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JGI_018131

    Plasmid

    #158935

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_119951226_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JGI_018133

    Plasmid

    #158936

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_101620581_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JGI_018135

    Plasmid

    #158937

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_096909276_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. JGI_018137

    Plasmid

    #158938

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_126985334_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. JGI_018139

    Plasmid

    #158939

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_058441655_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. JGI_018141

    Plasmid

    #158940

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    ABL00697_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. JGI_018143

    Plasmid

    #158941

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_061782733_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. JGI_018145

    Plasmid

    #158942

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_003805730_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. JGI_018147

    Plasmid

    #158943

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    OGN97459_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. JGI_018149

    Plasmid

    #158944

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_005508112_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. JGI_018151

    Plasmid

    #158945

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    CVN04163_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JGI_018153

    Plasmid

    #158946

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_005885358_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. JGI_018089

    Plasmid

    #158914

    Purpose

    Hydrocarbon Structures for Fuels and Chemicals

    Depositor

    Joint Genome Institute

    Insert

    WP_006592558_1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge