We narrowed to 2,853 results for: KIS;

1. hETFDH CRISPR_2

   Plasmid

   #232310

   Purpose

   Human ETFDH Gene Knockout (gRNA 2)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   ETFDH-targeting gRNA (ETFDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HR700_HA2TP53_Ex5

   Plasmid

   #228860

   Purpose

   Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5

   Depositor

   Thorsten Stiewe

   Article

   Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. HR700_HA2TP53_Ex6

   Plasmid

   #228861

   Purpose

   Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6

   Depositor

   Thorsten Stiewe

   Article

   Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. HR700_HA2TP53_Ex7

   Plasmid

   #228862

   Purpose

   Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7

   Depositor

   Thorsten Stiewe

   Article

   Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. HR700_HA2TP53_Ex8

   Plasmid

   #228863

   Purpose

   Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8

   Depositor

   Thorsten Stiewe

   Article

   Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pKM-U6-reRNA-hsRUNX

   Plasmid

   #205639

   Purpose

   reRNA guide targeting Homo sapiens RUNX

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-hsRUNX (RUNX1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNK189

   Plasmid

   #219743

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   D37E, V181I, S323M, M385K

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNK4169

   Plasmid

   #219744

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   D37E, V181I, S323M, M385K

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLX304_miRFP670nano-P2A-ERM-gCam6f

   Plasmid

   #214927

   Purpose

   Express miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)

   Depositor

   Xinnan Wang

   Article

   Bharat et al Cell Rep. 2023 Dec 26;42(12):113544. doi

   Insert

   miRFP670nano-P2A-ERM-gCam6f
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVp

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.1 puro shCALM2-1

    Plasmid

    #193699

    Purpose

    Constitutive lentiviral expression of CALM2 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    CALM2 (CALM2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO.1 puro shCALM2-2

    Plasmid

    #193700

    Purpose

    Constitutive lentiviral expression of CALM2 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    CALM2 (CALM2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO.1 puro shCALM2-3

    Plasmid

    #193701

    Purpose

    Constitutive lentiviral expression of CALM2 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    CALM2 (CALM2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCUU

    Plasmid

    #159747

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223 ATG3 C264A

    Plasmid

    #129293

    Purpose

    Gateway entry clone encoding human ATG3 C264A (catalytic inactive)

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    changed Cysteine 264 to Alanine

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223 ATG3 allKR

    Plasmid

    #129295

    Purpose

    Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    changed all 22 Lysine residues to Arginine

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV 3xFLAG-ATG3 allKR

    Plasmid

    #129298

    Purpose

    Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed all 22 Lysine residues to Arginine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV 3xFLAG-ATG3 1-13 KR

    Plasmid

    #129299

    Purpose

    Expresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed first 13 Lysine residues to Arginine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV 3xFLAG-ATG3 14-22 KR

    Plasmid

    #129300

    Purpose

    Expresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed last 9 Lysine residues to Arginine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV 3xFLAG-ATG3 K295R

    Plasmid

    #129302

    Purpose

    Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 295 to Arginine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5-LAP-MPS1-dTPR

    Plasmid

    #114048

    Purpose

    Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-dTPR (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta TPR (deletion of TPR domain 63-191)

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA5-LAP-MPS1-d60

    Plasmid

    #114046

    Purpose

    Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-d60 (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta 60 (deletion of NTE domain)

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. px330-Axin1

    Plasmid

    #162543

    Purpose

    sgRNA targeting Axin1

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Axin1 (Axin1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-Syn-DIO-PinkyCaMP

    Plasmid

    #232857

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNK497

    Plasmid

    #219754

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-ARAP3

    Plasmid

    #39484

    Depositor

    Sonja Vermeren

    Article

    Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

    Insert

    ARAP3 (ARAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pSF143-wArchon1-KGC-EGFP-ER2

    Plasmid

    #108429

    Purpose

    Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    wArchon1-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    flp-18

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-Archon1-KGC-EGFP-ER2-WPRE

    Plasmid

    #108423

    Purpose

    Archon1 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT2/shCol1a1/GFP4_Seq1.3

    Plasmid

    #206136

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-ALK6 Q203D

    Plasmid

    #80883

    Purpose

    mammalian expression of ALK6 Q203D

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK6 (Bmpr1b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q203D (constitutively active)

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA3-ALK6 K231R

    Plasmid

    #80884

    Purpose

    mammalian expression of ALK6 K231R

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK6 (Bmpr1b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K231R (kinase-deficient)

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pWZL-Neo-Myr-Flag-DEST

    Plasmid

    #15300

    Purpose

    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

    Depositor

    Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid

    #166693

    Purpose

    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and U6

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBabe-Puro-Myr-Flag-IKBKE

    Plasmid

    #15293

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Myr-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

35. pAAV-CaMKII-Archon1-EGFP

    Plasmid

    #108417

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHR-EF1a-Archon1-KGC-EGFP-ER2

    Plasmid

    #108425

    Purpose

    Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV 3xFLAG-LC3B Q116P G120

    Plasmid

    #129289

    Purpose

    Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBabe-Puro-Flag-IKBKE

    Plasmid

    #15292

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

39. pCAG-Archon2-KGC-GFP-ER2-WPRE

    Plasmid

    #108424

    Purpose

    Archon2 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon2-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBabe-Neo-Flag-IKBKE

    Plasmid

    #15265

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

41. pBabe-Puro-cRAF-W22

    Plasmid

    #15270

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    CRAF (RAF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

42. pmiRFP-N1

    Plasmid

    #108409

    Purpose

    Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    miRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

    Plasmid

    #216321

    Purpose

    Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Luciferase + splice acceptor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

    Plasmid

    #216322

    Purpose

    Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pTol2-UAS-zmiRFP

    Plasmid

    #108415

    Purpose

    Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zmiRFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBAD-HisD-miRFP

    Plasmid

    #108410

    Purpose

    Monomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    miRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHR-EF1a-Archon2-KGC-EGFP-ER2

    Plasmid

    #108426

    Purpose

    Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon2-KGC-EGFP-ER2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. GLDC-RES

    Plasmid

    #72882

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    GLDC (GLDC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    silent mutations in site corresponding to shGLDC_2

    Promoter

    CMV

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

49. pBabe-Neo-Myr-Flag-IKBKE

    Plasmid

    #15267

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Myr-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

50. MIG-eIF4A1 P159E

    Plasmid

    #70042

    Purpose

    Expression of eIF4A1 P159E mutant construct in mammalian cells.

    Depositor

    Hans-Guido Wendel

    Article

    Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

    Insert

    eIF4A1 (Eif4a1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    P159E

    Promoter

    CMV

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only