We narrowed to 2,847 results for: PHI-1
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Plasmid#134738PurposeLentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseLentiviralTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202PromoterCMVAvailable sinceOct. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold-TUBA1B
Plasmid#227321PurposeDonor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)DepositorInsertTUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 R57A, R59A pMT2
Plasmid#206112Purposeexpression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulationDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationR57A, R59APromoterAd MLP/TPL/SV40Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-moxGFP-TUBA1B
Plasmid#207766PurposeDonor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP Cav2.2 D122A pcDNA3
Plasmid#206068Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307Y-pKK223
Plasmid#131381PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.DepositorInsertEcNGsC MutY chimera F307Y
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307A-pKK223
Plasmid#131382PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308V-pKK223
Plasmid#131393PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.DepositorInsertEcNGsC MutY chimera S308V
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308A-pKK223
Plasmid#131394PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.DepositorInsertEcNGsC MutY chimera S308A
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral FL-15A
Plasmid#113011PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)DepositorInsertfull length tral (tral Fly)
UseTagsExpressionBacterialMutation15 PNG phosphorylation sites mutated to APromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C 15A
Plasmid#113007PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)DepositorInsertC terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationPNG phosphorylation sites mutated to APromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-623
Plasmid#92266PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cellsDepositorInsertdGW182 6W6A (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced W23…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-695
Plasmid#92263PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cellsDepositorInsertdGW182 PAM2 mut (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced: EF…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
Plasmid#207024PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384A Y387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP
Plasmid#207023PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371A I375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-Y387A-GFP
Plasmid#207022PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationY387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A-GFP
Plasmid#207021PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-I375A-GFP
Plasmid#207020PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationI375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A-GFP
Plasmid#207019PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ypq2-2GFP-URA
Plasmid#207010PurposeIntegration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertYPQ2
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Vph1-2GFP-URA
Plasmid#207009PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertVPH1
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8-MET15
Plasmid#207055PurposeExpression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationPromoterpATG8Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308P-H309D-pKK223
Plasmid#131378PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.DepositorInsertEcNGsC MutY chimera S308P H309D
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307A-S308A-pKK223
Plasmid#131379PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A S308A
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral FL-15D
Plasmid#113010PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutationsDepositorInsertfull length tral (tral Fly)
UseTagsExpressionBacterialMutation15 PNG phosphorylation sites mutated to DPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-646
Plasmid#92289PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cellsDepositorInsertdGW182 CED 7W7A (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215 with the following point …PromoterAc5Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
I-2/pCS2MT
Plasmid#16317DepositorInsertInhibitor-2 (Ppp1r2 Mouse)
UseVertebrate expressionTags5x mycExpressionMutationPromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOET3-6xHis-hSMSr-ΔSAMD
Plasmid#236225PurposeExpress N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cellsDepositorInsertSAMD8 (SAMD8 Human)
UseTagsHexa histidine tag (6xHis-tag) and enterokinase s…ExpressionInsectMutationsterile alpha motif domain at N-terminal (1-231 b…PromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYC01
Plasmid#224586PurposeIPTG-inducible phage φX174 lysis gene EDepositorInsertphage φX174 lysis gene E (phiX174p08 )
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPA1lacO-1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-calx1
Plasmid#58697PurposeExpression of calx1DepositorInsertcalx1 (Calx Fly)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
UAS-dar1-V5
Plasmid#45056DepositorInsertdar1 (dar1 Fly)
UseTagsV5ExpressionInsectMutationPromoterHS promoterAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
CJ47 (U1 snRNA (U1;21d) 3G mutant)
Plasmid#17776DepositorInsertU1 snRNA (U1;21d) (snRNA:U1:21D Fly)
UseTagsExpressionMutation3G mutantPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
OA-986F (AAEL003877-dCas9-VPR)
Plasmid#183993PurposeExpresses dCas9-VPR under the Ae. aegypti polyubiquitin promoterDepositorInsertAAEL003877-dCAS9-VPR
UseCRISPRTagsdCas9-VPRExpressionInsectMutationPromoterAAEL003887Available sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
UG76-pLYS2-GFP-PKCbeta
Plasmid#184762PurposeMarker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorInsertpLYS2-GFP-PKCbeta (PRKCB Budding Yeast, Human)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 ShhN
Plasmid#37680DepositorInsertshh (Shh Mouse)
UseTagsExpressionMammalianMutationsoluble form (see comments)PromoterCMVAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed
Plasmid#51434PurposeVector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.DepositorInsertLoxP-3xP3-DsRed-LoxP
UseTagsExpressionMutationPromoter3xP3Available sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCFJ1273
Plasmid#44487DepositorInsertMinimal Mos1, peel-1, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvH-CtPAN3_448-555_Y
Plasmid#148093PurposeBacterial Expression of CtPAN3_448-555DepositorInsertCtPAN3_448-555
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGex-CS-DmCup_296-425-L364AL368A-GB1_Z
Plasmid#148169PurposeBacterial Expression of DmCup_296-425-L364AL368ADepositorInsertDmCup_296-425-L364AL368A (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGex-CS-DmCup_296-425-Y327AL332A-GB1_Z
Plasmid#148170PurposeBacterial Expression of DmCup_296-425-Y327AL332ADepositorInsertDmCup_296-425-Y327AL332A (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGex-CS-DmCup_296-425-YLLLtoAAAA-GB1_Z
Plasmid#148171PurposeBacterial Expression of DmCup_296-425-YLLLtoAAAADepositorInsertDmCup_296-425-YLLLtoAAAA (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-CtPAN2_1-457_Y
Plasmid#148094PurposeBacterial Expression of CtPAN2_1-457DepositorInsertCtPAN2_1-457
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-CtPAN2opt_1-490_V
Plasmid#147787PurposeBacterial Expression of CtPAN2opt_1-490DepositorInsertCtPAN2opt_1-490
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-CtPAN3opt_171-566_V
Plasmid#147789PurposeBacterial Expression of CtPAN3opt_171-566DepositorInsertCtPAN3opt_171-566
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup-4Ala-mut_O
Plasmid#147100PurposeInsect Expression of DmCup-4Ala-mutDepositorInsertDmCup-4Ala-mut (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup-del580-600_O
Plasmid#147101PurposeInsect Expression of DmCup-del580-600DepositorInsertDmCup-del580-600 (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_57-684_J
Plasmid#146694PurposeInsect Expression of DmHPat_57-684DepositorInsertDmHPat_57-684 (Patr-1 Fly)
UseTagsExpressionInsectMutationTwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-del57-499_H
Plasmid#146460PurposeInsect Expression of DmHPat-del57-499DepositorInsertDmHPat-del57-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation C2340A compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only