We narrowed to 5,262 results for: 290

1. pKLV2-U6gRNA5(gKAT6A-A8)-PGKpuro2ABFP-W

   Plasmid

   #159290

   Purpose

   Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A8)

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   guide RNA targeting KAT6A (ID. A8) (KAT6A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBP-parallel1-GK5_v2 (D280N)

   Plasmid

   #134290

   Purpose

   Expresses GK5 (isoform 2)-D280N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 280 to Asparagine

   Promoter

   T7

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-pcDNA3.1 Puro-CAG-Voltron2

   Plasmid

   #172909

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_EGFR_p.L858R

   Plasmid

   #82906

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   L858R

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5-LRRK2

   Plasmid

   #229019

   Purpose

   Expression of untagged full length human LRRK2 in mammalian cells

   Depositor

   Samara Reck-Peterson

   Article

   Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Use

   Tags

   no tags (untagged)

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_PIK3CA_WT

   Plasmid

   #81736

   Purpose

   Gateway Donor vector containing PIK3CA , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_ARAF_WT

   Plasmid

   #82900

   Purpose

   Gateway Donor vector containing ARAF, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   NM_001654 (ARAF Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-KLF6

   Plasmid

   #192909

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of KLF6

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   KLF6 (KLF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-LETM1

   Plasmid

   #132290

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   LETM1 (LETM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pDONR223_PIK3CA_p.H1047Y

    Plasmid

    #81701

    Purpose

    Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    H1047Y

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_CDK2_WT

    Plasmid

    #82296

    Purpose

    Gateway Donor vector containing CDK2, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL3.7-EF-EYFP-YAP1_WW-PolyA

    Plasmid

    #112290

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDA

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with mutations in the WW domain…

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1962

    Plasmid

    #142900

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CUX1 (CUX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-cT3G-cERP2-KLF4

    Plasmid

    #192908

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of KLF4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_PIK3CA_p.D1045V

    Plasmid

    #81702

    Purpose

    Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    D1045V

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129048

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129041

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_ARAF_p.S214F

    Plasmid

    #82901

    Purpose

    Gateway Donor vector containing ARAF , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ARAF (ARAF Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S214F

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

    Plasmid

    #108290

    Purpose

    Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed L 188 to A, changed L 192 to A

    Promoter

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO_HSV.V5.HA_NGFR

    Plasmid

    #158290

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HSV.V5.HA

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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