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pSCALPS_miR-146aSponge_mCherry_GFP

Plasmid

#149718

Purpose

Lentiviral vector expressing a miR-146a decoy sponge

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

14X miR-146a binding sites

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G to T mutation in position 75 of the insert

Promoter

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 4

Plasmid

#36114

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B4 (Gnb4 Mouse)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

cmv

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
p-mCherry2-sgC9cen

Plasmid

#198326

Purpose

Expresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporter

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

Chr9-CEN guide RNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 3

Plasmid

#36113

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B3 (GNB3 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

cmv

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pDECKO_mCherry_Rat_cebp-alfa

Plasmid

#140098

Purpose

CRISPR-Cas9 library validation (positive control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

gRNA1 under U6 and gRNA2 under H1

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-VEGFA

Plasmid

#140588

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA VEGFA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neoBeta2mcherry

Plasmid

#45097

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

nAChR beta2 mcherry (Chrnb2 Mouse)

Use

Tags

has c-myc tag

Expression

Mammalian

Mutation

β2 was cloned into pCI-neo at the restriction sit…

Promoter

CMV

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pCD86-mCherry2

Plasmid

#190747

Purpose

Expression mCherry2-tagged CD86 in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTH-NI(A)-mCherry

Plasmid

#49626

Purpose

Destination Vector for TALEColor-mCherry

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Type

Empty backbone

Use

TALEN

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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p63(1-29)-mCherry-N1

Plasmid

#67895

Purpose

Expresses of fusion of mCherry with the N-terminal 29 amino acids of p63RhoGEF

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

p63RhoGEF(1-29)-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-Cx26-7

Plasmid

#55021

Purpose

Localization: Gap Junctions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Cx26

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
PA-mCherry1-PMP-N-10

Plasmid

#57195

Purpose

Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

PMP (PXMP2 Human)

Use

Tags

PA-mCherry1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only
pETM6-G6-mCherry

Plasmid

#66533

Purpose

Expresses mCherry under the control of Mutant T7 Promoter G6

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - G6

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR L3-mCherry-HA-L2

Plasmid

#32313

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

mCherry-HA

Use

Gateway multisite entry vector

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40

Plasmid

#100030

Purpose

AAV expression of ArcLightD

Depositor

Lawrence Cohen

Article

Storace et al Sci Rep. 2015 May 13;5:10212. doi: 10.10

Insert

ArcLightD

Use

AAV

Tags

2A-mCherry

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-ATG16-N-18

Plasmid

#54992

Purpose

Localization: Membrane Receptor, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ATG16

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-Talin-R-15

Plasmid

#55140

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Talin R

Use

Tags

mCherry

Expression

Mammalian

Mutation

Talin Rod Domain

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
PB-Haunt E1-PuroT2AmCherry-4A KI

Plasmid

#92164

Purpose

For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt P-Inr2-cDNA KI homology arms

Use

Other

Tags

Expression

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.S.VSVg_mCherry-NLS

Plasmid

#178269

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.S.VSVg and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.HSV.VSVg_mCherry-NLS

Plasmid

#178261

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.HSV.VSVg and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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