We narrowed to 5,331 results for: pcdna3.1

1. pcDNA3.1+_V5-ANKRD52

   Plasmid

   #92012

   Purpose

   Expresses V5-ANKRD52

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   ANKRD52 (ANKRD52 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY129 (pcDNA3.1-U6-huMb3Cpf1)

   Plasmid

   #92305

   Purpose

   Expression of humanized Mb3Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb3Cpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CibN-dCas9

   Plasmid

   #60551

   Purpose

   Expresses CibN-dCas9 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   CibN-dCas9
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/V5-His-TN-DROSHA

   Plasmid

   #106252

   Purpose

   Express TN-DROSHA in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

   Insert

   Drosha Ribonuclease III (DROSHA Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   E1045Q and E1222Q mutations

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MS2-HA-HsNot4_AA

   Plasmid

   #148255

   Purpose

   Mammalian Expression of HsNot4

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   HsNot4 (CNOT4 Human)
   

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY127 (pcDNA3.1-U6-huTsCpf1)

   Plasmid

   #92303

   Purpose

   Expression of humanized TsCpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huTsCpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+) Drp1 V2 S579D

   Plasmid

   #46343

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   Drp1 V2 (Dnm1l Mouse)
   

   Expression

   Mammalian

   Mutation

   S579D

   Promoter

   CMV

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-Firefly-ccdB

   Plasmid

   #87069

   Purpose

   Mammalian Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

   Plasmid

   #67299

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   Tiam1-pMagFast2(3x)-iRFP (Tiam1 Synthetic, Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 EYFP H148Q

    Plasmid

    #25871

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q
    

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with Histidine 148 to …

    Available Since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid

    #181857

    Purpose

    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-JNKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid

    #181856

    Purpose

    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AktAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) Drp1 V2 S579A

    Plasmid

    #46342

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    Drp1 V2 (Dnm1l Mouse)
    

    Expression

    Mammalian

    Mutation

    S579A

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-HisFlag-CAD

    Plasmid

    #31615

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

    Insert

    CAD
    

    Tags

    3xFlag, His, and T7

    Expression

    Mammalian

    Available Since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid

    #126472

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-Mito-CaNARi

    Plasmid

    #64851

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Tags

    Cerulean, N-terminal 30 amino acids from DAKAP1, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-Golgi-RasAR

    Plasmid

    #205570

    Purpose

    Golgi membrane-targeted RasAR.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    Golgi-RasAR (RAF1, GOLGB1 Synthetic, Human)
    

    Tags

    Golgin B1 (Giantin) residues 3131-3259

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_PA-Cre-Y324F

    Plasmid

    #122961

    Purpose

    Catalytically inactive mutant of PA-Cre

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_PA-Cre-Y324F
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-myc-AP1S3

    Plasmid

    #58292

    Purpose

    mammalian expression of AP1S3

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Tags

    6xHis and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-hFluc-V5-6xbsMS2_U

    Plasmid

    #147639

    Purpose

    Mammalian Expression of 6xbsMS2

    Depositor

    Elisa Izaurralde

    Insert

    6xbsMS2
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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