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pYSD099

Plasmid

#212923

Purpose

A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

GFP

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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fabp10a:H2B-mGreenLantern

Plasmid

#230045

Purpose

Expression of nuclear green fluorescent protein in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

Insert

mGreenLantern

Use

Zebrafish expression

Tags

H2B

Promoter

fabp10a

Available Since

Feb. 5, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEK36

Plasmid

#210598

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter

Tags

mClover3

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-6085x

Plasmid

#108653

Purpose

A tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

PicchuEV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTSAR3.5t

Plasmid

#210257

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

eBFP2
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_sfGPF 150TAG

Plasmid

#154765

Purpose

plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTiGc

Plasmid

#78314

Purpose

Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).

Depositor

Samantha Sampson

Article

Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/

Inserts

TurboFP635
GFP

Use

Synthetic Biology; Mycobacterial replicating vect…

Expression

Bacterial

Promoter

hsp60 and hsp60(ribo)

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pTLR repair vector

Plasmid

#64322

Purpose

Vector for repair of the traffic light reporter (TLR), providing the venus coding region

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

Venus

Use

Artificial targeting vector

Mutation

see publication for details

Promoter

none

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN173

Plasmid

#22012

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bJunVN173 (Jun Aequorea Victoria, Rat)

Tags

FLAG

Expression

Mammalian

Mutation

c-Jun(257-338)-Venus(1-172)

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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ins:CFP-NTR

Plasmid

#66593

Purpose

Specific and conditional cell ablation of beta cells in the zebrafish pancreas

Depositor

Didier Stainier

Article

Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.

Insert

Insulin promotor driving CFP-nitroreductase

Use

Zebrafish expression

Tags

CFP fused with N-terminus of nitroreductase

Promoter

Zebrafish Insulin promoter (approximately 1.2kb)

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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CTV

Plasmid

#15912

Depositor

Klaus Rajewsky

Article

Thai et al Science. 2007 Apr 27. 316(5824):604-8.

Insert

CAG-STOP-eGFP-ROSA26TV

Use

Cre/Lox; /stop

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pv6_EGFP

Plasmid

#179392

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of EGFP

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

EGFP

Tags

AviTag

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6015nes

Plasmid

#108657

Purpose

A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-S6K-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex-GFP

Plasmid

#197883

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFEndoNA

Plasmid

#174724

Purpose

Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

Depositor

Jukka Finne

Article

Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

Insert

GFP-endoNA fusion protein

Tags

Histag

Expression

Bacterial

Promoter

T5

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pME-nls-sfGFP

Plasmid

#132973

Purpose

full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

nuclear localized super folder GFP

Use

Gateway vector

Tags

6x myc and SV40 NLS

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSIQmate-EGFP

Plasmid

#216241

Purpose

All-in One IQ-Cumate transgene switch for the EGFP expression

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-GFP

Plasmid

#190231

Purpose

AAV vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pL0_CD-greenlantern

Plasmid

#202141

Purpose

Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Green lantern gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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013LP-pAAV-ANF-657-EmGFP-pA

Plasmid

#120267

Purpose

AAV vector for Atrial specific EmGFP expression

Depositor

Xander Wehrens

Article

Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

Insert

EmGFP

Use

AAV

Tags

3XFlag and HA

Promoter

ANF promoter

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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